Macromolecular Chemistry and Bavarian Polymer Institute, University of Bayreuth, Universitätsstrasse 30, 95440, Bayreuth, Germany.
Bavarian Center for Battery Technology (BayBatt) and Department of Chemistry, University of Bayreuth, Universitätsstrasse 30, 95440, Bayreuth, Germany.
Macromol Biosci. 2023 Nov;23(11):e2300143. doi: 10.1002/mabi.202300143. Epub 2023 Jul 6.
Biodegradable electrospun sponges are of interest for various applications including tissue engineering, drug release, dental therapy, plant protection, and plant fertilization. Biodegradable electrospun poly(l-lactide)/poly(ε-caprolactone) (PLLA/PCL) blend fiber-based sponge with hierarchical pore structure is inherently hydrophobic, which is disadvantageous for application in tissue engineering, fertilization, and drug delivery. Contact angles and model studies for staining with a hydrophilic dye for untreated, plasma-treated, and surfactant-treated PLLA/PCL sponges are reported. Thorough hydrophilization of PLLA/PCL sponges is found only with surfactant-treated sponges. The MTT assay on the leachates from the sponges does not indicate any cell incompatibility. Furthermore, the cell proliferation and penetration of the hydrophilized sponges are verified by in vitro cell culture studies using MG63 and human fibroblast cells.
可生物降解的静电纺丝海绵因其在组织工程、药物释放、牙科治疗、植物保护和植物施肥等领域的各种应用而受到关注。具有分级孔结构的可生物降解静电纺丝聚（L-丙交酯）/聚（ε-己内酯）（PLLA/PCL）共混纤维基海绵本质上是疏水的，这不利于其在组织工程、施肥和药物输送中的应用。报告了未处理、等离子体处理和表面活性剂处理的 PLLA/PCL 海绵的接触角和用亲水性染料染色的模型研究。只有经过表面活性剂处理的海绵才能实现 PLLA/PCL 海绵的彻底亲水化。海绵浸出液的 MTT 测定表明没有细胞不相容性。此外，通过使用 MG63 和人成纤维细胞的体外细胞培养研究验证了亲水化海绵的细胞增殖和渗透。
