Macromolecular Chemistry and Bavarian Polymer Institute , University of Bayreuth , Universitätsstrasse 30 , 95440 Bayreuth , Germany.
Process Biotechnology , University of Bayreuth , 95440 , Bayreuth , Germany.
Biomacromolecules. 2018 May 14;19(5):1663-1673. doi: 10.1021/acs.biomac.8b00434. Epub 2018 Apr 10.
Ultraporous, degradable sponges made of either polylactide or of blends of polylactide/poly(ε-caprolactone) are prepared by freeze-drying of dispersions of short electrospun fibers and subsequent thermal annealing. The sponges feature ultrahigh porosity (99.6%), a hierarchical cellular structure, and high reversible compressibility with fast recovery from deformation in the dry as well as in the wet state. The sponge properties depend on the fiber dispersion concentration and the annealing temperature. Sponge characteristics like fiber density (2.5-20 mg/cm), size, shape, crystallinity, mechanical strength, wetability, and structural integrity are user adjustable. Cell culture experiments were successfully performed with Jurkat cells with Confocal Laser Scanning Microscopy and MTT staining showing rapid cell proliferation. Live/Dead staining demonstrated high viability of the seeded cells. The sponge characteristics and modifications investigated and presented here reveal that these sponges are highly promising for tissue engineering applications.
由聚乳酸或聚乳酸/聚（ε-己内酯）共混物制成的超多孔、可降解的海绵，是通过短电纺纤维分散体的冷冻干燥和随后的热退火制备的。这些海绵具有超高孔隙率（99.6%）、分级多孔结构和高的可压缩性，在干燥和湿润状态下都能快速从变形中恢复。海绵的性能取决于纤维分散浓度和退火温度。像纤维密度（2.5-20mg/cm）、大小、形状、结晶度、机械强度、润湿性和结构完整性等海绵特性都是可调节的。使用共聚焦激光扫描显微镜和 MTT 染色对 Jurkat 细胞进行细胞培养实验，结果表明细胞快速增殖。活/死染色表明接种细胞具有高活力。这里研究和提出的海绵特性和改性表明，这些海绵非常有希望用于组织工程应用。
