Tecnologico Nacional de México, Instituto Tecnológico de Tuxtla Gutiérrez, Tuxtla Gutiérrez, Chiapas, México.
Departamento de Bioquímica, Instituto Nacional de Cardiología Ignacio Chávez, Mexico City, México.
Curr Microbiol. 2023 Jun 26;80(8):257. doi: 10.1007/s00284-023-03327-8.
The crater lake at "El Chichón" volcano is an extreme acid-thermal environment with high concentrations of heavy metals. In this study, two bacterial strains with the ability to resist high concentrations of arsenic (As) were isolated from water samples from the crater lake. Staphylococcus ARSC1-P and Stenotrophomonas ARSC2-V isolates were identified by use of the 16S rDNA gene. Staphylococcus ARSC1-P was able to grow in 400 mM of arsenate [As(V)] under oxic and anoxic conditions. The IC values were 36 and 382 mM for oxic and anoxic conditions, respectively. For its part, Stenotrophomonas ARSC2-V showed IC values of 110 mM and 2.15 for As(V) and arsenite [As(III)], respectively. Arsenic accumulated intracellularly in both species [11-25 nmol As × mg cellular prot in cells cultured in 50 mM As(V)]. The present study shows evidence of microbes that can potentially be a resource for the bio-treatment of arsenic in contaminated sites, which highlights the importance of the "El Chichón" volcano as a source of bacterial strains that are adaptable to extreme conditions.
“埃尔奇琼”火山的火山口湖是一个极端的酸热环境，含有高浓度的重金属。在这项研究中，从火山口湖的水样中分离到了两株具有抗高浓度砷（As）能力的细菌菌株。通过使用 16S rDNA 基因，鉴定出葡萄球菌 ARSC1-P 和寡养单胞菌 ARSC2-V 两种分离物。葡萄球菌 ARSC1-P 能够在有氧和缺氧条件下生长在 400mM 的砷酸盐 [As(V)]中。在有氧和缺氧条件下，IC 值分别为 36 和 382mM。就其本身而言，寡养单胞菌 ARSC2-V 对 As(V)和亚砷酸盐 [As(III)]的 IC 值分别为 110mM 和 2.15。两种物质在细胞内都积累了砷[在 50mMAs(V)培养的细胞中，细胞内的砷含量为 11-25nmolAs×mg 细胞蛋白]。本研究表明，微生物有可能成为受污染地点砷生物处理的资源，这凸显了“埃尔奇琼”火山作为适应极端条件的细菌菌株来源的重要性。
