Ministry of Education Key Laboratory for Cross-Scale Micro and Nano Manufacturing, Changchun University of Science and Technology, Changchun, China.
Institute for Research in Applicable Computing, University of Bedfordshire, Luton, UK.
J Microsc. 2023 Sep;291(3):229-236. doi: 10.1111/jmi.13212. Epub 2023 Jul 12.
Many diseases are related to changes in the biomechanical properties of cells; their study can provide a theoretical basis for drug screening and can explain the internal working of living cells. In this study, the biomechanical properties of nephrocytes (VERO cells), hepatocytes (HL-7702 cells), and hepatoma cells (SMCC-7721 cells) in culture were detected by atomic force microscopy (AFM) to analyse the side effects of colchicine at different concentrations (0.1 μg/mL (A) and 0.2 μg/mL (B)) at the nanoscale for 2, 4 and 6 h. Compared with the corresponding control cells, the damage to the treated cells increased in a dose-dependent manner. Among normal cells, the injury of nephrocytes (VERO cells) was markedly worse than that of hepatocytes (HL-7702 cells) in both colchicine solutions A and B. Based on the analyses of biomechanical properties, the colchicine solution reduced the rate of division and inhibited metastasis of SMCC-7721 cells. By comparing these two concentrations, we found that the anticancer effect of colchicine solution A was greater than that of solution B. Studying the mechanical properties of biological cells can help understand the mechanism of drug action at the molecular level and provide a theoretical basis for preventing the emergence and diagnosis of diseases at the nanoscale.
许多疾病都与细胞生物力学特性的变化有关；它们的研究可为药物筛选提供理论基础，并能解释活细胞的内部工作原理。在这项研究中，我们通过原子力显微镜（AFM）检测了培养中的肾细胞（VERO 细胞）、肝细胞（HL-7702 细胞）和肝癌细胞（SMCC-7721 细胞）的生物力学特性，以分析不同浓度（0.1μg/mL（A）和 0.2μg/mL（B））秋水仙素在纳米尺度下对细胞的影响，作用时间分别为 2、4 和 6 小时。与相应的对照细胞相比，处理细胞的损伤呈剂量依赖性增加。在正常细胞中，两种秋水仙素溶液 A 和 B 处理的肾细胞（VERO 细胞）的损伤明显比肝细胞（HL-7702 细胞）严重。基于生物力学特性的分析，秋水仙素溶液降低了 SMCC-7721 细胞的分裂率并抑制了其转移。通过比较这两种浓度，我们发现秋水仙素溶液 A 的抗癌效果大于溶液 B。研究生物细胞的机械特性有助于在分子水平上理解药物作用的机制，并为预防疾病在纳米尺度上的出现和诊断提供理论依据。
