Liu Yang, Wang Zuobin, Wang Xinyue
CNM & JR3CN Centres, Changchun University of Science and Technology Changchun, China.
CNM & JR3CN Centres, Changchun University of Science and Technology Changchun, China.
J Mech Behav Biomed Mater. 2015 May;45:65-74. doi: 10.1016/j.jmbbm.2014.12.011. Epub 2014 Dec 18.
In this study, the alterations of the morphology and biomechanical properties of living SMCC-7721 cancer cells treated with fullerenol (C60(OH)24) for 24, 48, and 72h were investigated using an atomic force microscope (AFM). Comparative analyses show that the elastic moduli of the SMCC-7721 cells exposed to fullerenol decrease significantly with the increase of the treatment periods. Furthermore, in different phases of the treatment, a global decrease in elasticity is accompanied by cellular morphological changes, and the time-dependent effect of the fullerenol can be observed using AFM and optical microscope. In addition, as the treatment duration increases, the indentation force and depth penetrated into the cell membrane by the AFM tip are in a declining trend. The reduction in the stiffness of the cells exposed to fullerenol could be associated with the disruption of the cellular cytoskeleton network. The investigation indicates that the elastic modulus of single living cells can be a useful biomarker to evaluate the effects of fullerenol or other anticancer agents on the cells and reveal instructive information for cellular dynamic behaviors.
在本研究中，使用原子力显微镜（AFM）研究了用富勒醇（C60(OH)24）处理24、48和72小时的活SMCC-7721癌细胞的形态和生物力学特性的变化。对比分析表明，暴露于富勒醇的SMCC-7721细胞的弹性模量随着处理时间的增加而显著降低。此外，在处理的不同阶段，弹性的整体降低伴随着细胞形态的变化，并且使用AFM和光学显微镜可以观察到富勒醇的时间依赖性效应。另外，随着处理持续时间的增加，AFM针尖压入细胞膜的压痕力和深度呈下降趋势。暴露于富勒醇的细胞刚度降低可能与细胞骨架网络的破坏有关。该研究表明，单个活细胞的弹性模量可以作为一种有用的生物标志物，用于评估富勒醇或其他抗癌剂对细胞的影响，并揭示细胞动态行为的指导信息。
