College of Agriculture, Guizhou University, Guiyang, 550025, China.
Industry Technology Research Academy of Pepper, Guizhou University, Guiyang, 550025, China.
Mol Biol Rep. 2023 Aug;50(8):6655-6668. doi: 10.1007/s11033-023-08587-y. Epub 2023 Jun 26.
Pepper (Capsicum annum L.) is the main crop in the vegetable industry. The growth and development of peppers are regulated by nitrate, but there is limited research on the molecular mechanisms of nitrate absorption and assimilation in peppers. A plant specific transcription factor NLP plays an important role in nitrate signal transduction.
In this study, a total of 7 NLP members were identified based on pepper genome data. Two nitrogen transport elements (GCN4) were found in the CaNLP5 promoter. In the phylogenetic tree, CaNLP members are divided into three branches, with pepper NLP and tomato NLP having the closest genetic relationship. The expression levels of CaNLP1, CaNLP3, and CaNLP4 are relatively high in the roots, stems, and leaves. The expression level of CaNLP7 gene is relatively high during the 5-7 days of pepper fruit color transformation. After various non-Biotic stress and hormone treatments, the expression of CaNLP1 was at a high level. The expression of CaNLP3 and CaNLP4 was down regulated in leaves, but up regulated in roots. Under conditions of nitrogen deficiency and sufficient nitrate, the expression patterns of NLP genes in pepper leaves and roots were determined.
These results provide important insights into the multiple functions of CaNLPs in regulating nitrate absorption and transport.
辣椒（Capsicum annum L.）是蔬菜产业中的主要作物。辣椒的生长发育受硝酸盐调控，但对其硝酸盐吸收和同化的分子机制研究有限。植物特异性转录因子 NLP 在硝酸盐信号转导中发挥重要作用。
本研究基于辣椒基因组数据共鉴定出 7 个 NLP 成员。在 CaNLP5 启动子中发现了两个氮转运元件（GCN4）。在系统发育树中，CaNLP 成员分为三个分支，其中辣椒 NLP 和番茄 NLP 具有最密切的遗传关系。CaNLP1、CaNLP3 和 CaNLP4 在根、茎和叶中的表达水平相对较高。CaNLP7 基因在辣椒果实颜色转变的第 5-7 天表达水平较高。经过各种非生物胁迫和激素处理后，CaNLP1 的表达水平较高。CaNLP3 和 CaNLP4 在叶片中的表达下调，但在根部上调。在氮缺乏和充足硝酸盐条件下，确定了辣椒叶片和根部 NLP 基因的表达模式。
这些结果为 CaNLPs 调节硝酸盐吸收和转运的多种功能提供了重要见解。
