Guangdong Academy of Forestry, Guangzhou 510520, China.
Guangzhou Institute of Environmental Protection Science, Guangzhou 510520, China.
Genes (Basel). 2024 Aug 22;15(8):1108. doi: 10.3390/genes15081108.
Fire-resistant tree species play a crucial role in forest fire prevention, utilizing several physiological and molecular mechanisms to respond to extreme heat stress. Many transcription factors (TFs) and genes are known to be involved in the regulatory network of heat stress response in plants. However, their roles in response to high temperatures induced by fire remain less understood. In this study, we investigated , a fire-resistant tree, to elucidate these mechanisms. Leaves of seedlings were exposed to fire stimulation for 10 s, 30 s, and 1 min, followed by a 24-h recovery period. Fifteen transcriptomes were assembled to identify key molecular and biological pathways affected by high temperatures. Differentially expressed genes (DEGs) analysis revealed essential candidate genes and TFs involved in the heat stress response, including members of the ethylene-responsive factors, WRKY, MYB, bHLH, and Nin-like families. Genes related to heat shock proteins/factors, lipid metabolism, antioxidant enzymes, dehydration responses, and hormone signal transduction were differentially expressed after heat stress and recovery, underscoring their roles in cellular process and recovery after heat stress. This study advances our understanding of plant response and defense strategies against extreme abiotic stresses.
耐火树种在森林火灾预防中起着至关重要的作用，它们利用几种生理和分子机制来应对极端热应激。许多转录因子（TFs）和基因被认为参与了植物热应激反应的调控网络。然而，它们在应对火灾引起的高温方面的作用仍知之甚少。在这项研究中，我们研究了一种耐火树种，以阐明这些机制。将 幼苗的叶片暴露于火焰刺激 10 秒、30 秒和 1 分钟，然后进行 24 小时的恢复。共组装了 15 个转录组，以鉴定受高温影响的关键分子和生物学途径。差异表达基因（DEGs）分析揭示了参与热应激反应的重要候选基因和 TFs，包括乙烯响应因子、WRKY、MYB、bHLH 和 Nin-like 家族的成员。热应激和恢复后，与热休克蛋白/因子、脂质代谢、抗氧化酶、脱水反应和激素信号转导相关的基因表达差异，强调了它们在细胞过程和热应激恢复中的作用。这项研究增进了我们对植物应对和防御极端非生物胁迫策略的理解。
