Yamayoshi Seiya, Ito Mutsumi, Iwatsuki-Horimoto Kiyoko, Yasuhara Atsuhiro, Okuda Moe, Hamabata Taiki, Murakami Jurika, Duong Calvin, Yamamoto Tsukasa, Kuroda Yudai, Maeda Ken, Kawaoka Yoshihiro
Division of Virology, Institute of Medical Science, University of Tokyo, Japan.
International Research Center for Infectious Diseases, Institute of Medical Science, University of Tokyo, Japan.
One Health. 2023 Dec;17:100588. doi: 10.1016/j.onehlt.2023.100588. Epub 2023 Jun 19.
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has continued to circulate in humans since its emergence in 2019. While infection in humans continues, numerous spillover events to at least 32 animal species, including companion and zoo animals, have been reported. Since dogs and cats are highly susceptible to SARS-CoV-2 and have direct contact with their owners and other household members, it is important to know the prevalence of SARS-CoV-2 in dogs and cats. Here, we established an ELISA to detect serum antibodies against the receptor-binding domain and the ectodomain of the SARS-CoV-2 spike and nucleocapsid proteins. Using this ELISA, we assessed seroprevalence in 488 dog serum samples and 355 cat serum samples that were collected during the early pandemic period (between May and June of 2020) and 312 dog serum samples and 251 cat serum samples that were collected during the mid-pandemic period (between October 2021 and January 2022). We found that two dog serum samples (0.41%) collected in 2020, one cat serum sample (0.28%) collected in 2020, and four cat serum samples (1.6%) collected in 2021 were positive for antibodies against SARS-CoV-2. No dog serum samples collected in 2021 were positive for these antibodies. We conclude that the seroprevalence of SARS-CoV-2 antibodies in dogs and cats in Japan is low, suggesting that these animals are not a major SARS-CoV-2 reservoir.
严重急性呼吸综合征冠状病毒2（SARS-CoV-2）自2019年出现以来一直在人类中传播。尽管人类感染仍在持续，但已有大量向至少32种动物物种溢出传播的事件报道，包括伴侣动物和动物园动物。由于狗和猫对SARS-CoV-2高度易感，且与它们的主人和其他家庭成员有直接接触，了解狗和猫中SARS-CoV-2的流行情况很重要。在此，我们建立了一种酶联免疫吸附测定（ELISA）来检测针对SARS-CoV-2刺突蛋白和核衣壳蛋白的受体结合域及胞外域的血清抗体。利用这种ELISA，我们评估了在疫情早期（2020年5月至6月）采集的488份狗血清样本和355份猫血清样本，以及在疫情中期（2021年10月至2022年1月）采集的312份狗血清样本和251份猫血清样本中的血清阳性率。我们发现，2020年采集的两份狗血清样本（0.41%）、2020年采集的一份猫血清样本（0.28%）以及2021年采集的四份猫血清样本（1.6%）对SARS-CoV-2抗体呈阳性。2021年采集的狗血清样本中没有这些抗体呈阳性的。我们得出结论，日本狗和猫中SARS-CoV-2抗体的血清阳性率较低，这表明这些动物不是SARS-CoV-2的主要储存宿主。
