Gonçalves Ana Carolina Aparecida, Conzentino Marcelo Dos Santos, de Souza Barbosa Altina Bruna, Doline Fernando Rodrigo, Nilsson Mariana Guimarães, da Silva Aristeu Vieira, Kmetiuk Louise Bach, Biondo Alexander Welker, Huergo Luciano Fernandes
Seashore Campus Setor Litoral, Federal University of Paraná (UFPR), 512 Jaguariaíva Street, Matinhos, Paraná, 83260-000, Brazil.
Graduate College of Cellular and Molecular Biology, Federal University of Paraná (UFPR), Curitiba, Paraná, 81531-970, Brazil.
Braz J Microbiol. 2024 Dec;55(4):4183-4189. doi: 10.1007/s42770-024-01518-4. Epub 2024 Sep 20.
The fact that SARS-CoV-2 has reportedly infected companion, livestock and wildlife animals may constitute a significant risk for virus reservoirs, ground for emerging variants and potential for novel reverse zoonosis. Hence, SARS-CoV-2 surveillance in animal species is crucial to prevent emerging variants which may spread to humans. The present study aimed to develop a simple, high-throughput and ultrafast magnetic bead immunoassay to detect anti-SARS-CoV-2 nucleocapsid and spike reactive IgG antibodies in dog and cat serum samples. The assays were validated using serum from eleven dogs and cats which had SARS-CoV-2 infections confirmed by real-time RT-PCR. The negative cohort consisted of pre-pandemic dog and cat samples. The assays performed at 73-82% sensitivity and 97.5-98% specificity for dogs and 71% sensitivity and 92-94% specificity for cats. The lower assay specificity for cats is explained by the fact that cat pre-pandemic sera showed high levels of cross-reactive with SARS-CoV-2 Nucleocapsid and Spike, supporting that these animals have been exposed to other coronavirus sharing structural similarities with SARS-CoV-2. These assays described in this work are now being used for SARS-CoV-2 surveillance and research purposes.
据报道，严重急性呼吸综合征冠状病毒2（SARS-CoV-2）已感染伴侣动物、家畜和野生动物，这可能对病毒宿主构成重大风险，成为新变种出现的原因以及新型反向人畜共患病的潜在因素。因此，对动物物种进行SARS-CoV-2监测对于预防可能传播给人类的新变种至关重要。本研究旨在开发一种简单、高通量且超快速的磁珠免疫测定法，以检测犬猫血清样本中抗SARS-CoV-2核衣壳和刺突反应性IgG抗体。使用经实时RT-PCR确诊感染SARS-CoV-2的11只犬猫的血清对该测定法进行了验证。阴性对照包括疫情前的犬猫样本。该测定法对犬的敏感性为73-82%，特异性为97.5-98%；对猫的敏感性为71%，特异性为92-94%。猫的测定法特异性较低，原因是疫情前猫的血清与SARS-CoV-2核衣壳和刺突显示出高水平的交叉反应，这表明这些动物曾接触过与SARS-CoV-2结构相似的其他冠状病毒。本研究中描述的这些测定法目前正用于SARS-CoV-2监测和研究目的。
