Tang Ruihua, Xie Mingyue, Yan Xueyan, Qian Liwei, Giesy John P, Xie Yuwei
College of Bioresources Chemical and Materials Engineering, Shaanxi University of Science and Technology, Xi'an, 710021 People's Republic of China.
National Demonstration Center for Experimental Light Chemistry Engineering Education, Shaanxi University of Science and Technology, Xi'an, 710021 People's Republic of China.
Cellulose (Lond). 2023 Jun 3:1-13. doi: 10.1007/s10570-023-05288-4.
Nitrocellulose (NC) membrane was fabricated and tested for its potential use in various paper-based biosensors for use in point-of-care testing. However, contemporary technologies are complex, expensive, non-scalable, limited by conditions, and beset with potentially adverse effects on the environment. Herein, we proposed a simple, cost-effective, scalable technology to prepare nitrocellulose/cotton fiber (NC/CF) composite membranes. The NC/CF composite membranes with a diameter of 20 cm were fabricated in 15 min using papermaking technology, which contributes to scalability in the large-scale production of these composites. Compared with existing commercial NC membranes, the NC/CF composite membrane is characterized by small pore size (3.59 ± 0.19 μm), low flow rate (156 ± 55 s/40 mm), high dry strength (up to 4.04 MPa), and wet strength (up to 0.13 MPa), adjustable hydrophilic-hydrophobic (contact angles ranged from 29 ± 4.6 to 82.8 ± 2.4°), the good adsorption capacity of protein (up to 91.92 ± 0.07 μg). After lateral flow assays (LFAs) detection, the limit of detection is 1 nM, which is similar to commercial NC membrane (Sartorius CN 140). We envision the NC/CF composite membrane as a promising material for paper-based biosensors of point-of-care testing applications.
制备了硝酸纤维素（NC）膜，并测试了其在各种即时检测用纸质生物传感器中的潜在用途。然而，当代技术复杂、昂贵、不可扩展、受条件限制，且可能对环境产生不利影响。在此，我们提出了一种简单、经济高效且可扩展的技术来制备硝酸纤维素/棉纤维（NC/CF）复合膜。采用造纸技术在15分钟内制备出直径为20厘米的NC/CF复合膜，这有助于这些复合材料的大规模生产实现可扩展性。与现有的商用NC膜相比，NC/CF复合膜的特点是孔径小（3.59±0.19μm）、流速低（156±55秒/40毫米）、干强度高（高达4.04兆帕）和湿强度高（高达0.13兆帕），亲水-疏水性可调节（接触角范围为29±4.6至82.8±2.4°），蛋白质吸附能力良好（高达91.92±0.07微克）。经过侧向流动分析（LFA）检测，检测限为1纳摩尔，与商用NC膜（赛多利斯CN 140）相似。我们设想NC/CF复合膜是即时检测应用中纸质生物传感器的一种有前途的材料。
