State Key Laboratory of North China Crop Improvement and Regulation/ Key Laboratory of North China Water-saving Agriculture, Ministry of Agriculture and Rural Affairs/Key Laboratory of Crop Growth Regulation of Hebei Province/College of Agronomy, Hebei Agricultural University, Baoding, Hebei, China.
Cotton Research Institute, Hebei Academy of Agriculture and Forestry Sciences/Key Laboratory of Cotton Biology and Genetic Breeding in Huanghuaihai Semi-Arid Region, Ministry of Agriculture /Hebei Branch of National Cotton Improvement Center, Shijiazhuang, Hebei, China.
PeerJ. 2023 Jun 21;11:e15587. doi: 10.7717/peerj.15587. eCollection 2023.
Potassium plays a significant role in the basic functions of plant growth and development. Potassium uptake is closely associated with morphological characteristics of the roots. However, the dynamic characteristics of phenotype and lifespan of cotton ( L.) lateral roots and root hairs under low and high potassium stress remain unclear. In this study, potassium stress experiments (low and high potassium, medium potassium as control) were conducted using RhizoPot (an root observation device) to determine the response characteristics of lateral roots and root hairs in cotton under potassium stress. The plant morphology, photosynthetic characteristics, root phenotypic changes, and lifespan of lateral roots and root hairs were measured. Potassium accumulation, aboveground phenotype, photosynthetic capacity, root length density, root dry weight, root diameter, lateral root lifespan, and root hair lifespan under low potassium stress were significantly decreased compared to medium potassium treatment. However, the root hair length of the former was significantly increased than that of the latter. Potassium accumulation and the lateral root lifespan were significantly increased under high potassium treatment, while root length density, root dry weight, root diameter, root hair length, and root hair lifespan were significantly decreased compared to the medium potassium treatment. Notably, there were no significant differences in aboveground morphology and photosynthetic characters. Principal component analysis revealed that lateral root lifespan, root hair lifespan of the first lateral root, and root hair length significantly correlated with potassium accumulation. The root had similar regularity responses to low and high potassium stress except for lifespan and root hair length. The findings of this study enhance the understanding of the phenotype and lifespan of cotton's lateral roots and root hairs under low and high potassium stress.
钾在植物生长发育的基本功能中起着重要作用。钾的吸收与根的形态特征密切相关。然而，低钾和高钾胁迫下棉花（Gossypium hirsutum L.）侧根和根毛的表型和寿命的动态特征尚不清楚。本研究采用 RhizoPot（一种根系观察装置）进行钾胁迫实验（低钾和高钾，中钾作为对照），以确定钾胁迫下棉花侧根和根毛的响应特征。测量了植物形态、光合特性、根系表型变化以及侧根和根毛的寿命。与中钾处理相比，低钾胁迫下棉花的钾积累、地上形态、光合能力、根长密度、根干重、根直径、侧根寿命和根毛寿命显著降低，但前者的根毛长度显著增加。高钾处理下钾积累和侧根寿命显著增加，而根长密度、根干重、根直径、根毛长度和根毛寿命与中钾处理相比显著降低。值得注意的是，地上形态和光合特性没有显著差异。主成分分析表明，侧根寿命、第一侧根根毛寿命和根毛长度与钾积累显著相关。根对低钾和高钾胁迫的响应规律相似，除了寿命和根毛长度外。本研究的结果增强了对低钾和高钾胁迫下棉花侧根和根毛表型和寿命的理解。
