Guerrero I, Villasante A, Diamond L, Berman J W, Newcomb E W, Steinberg J J, Lake R, Pellicer A
Leuk Res. 1986;10(7):851-8. doi: 10.1016/0145-2126(86)90306-1.
Thymic lymphomas have been induced by gamma-radiation and treatment with the chemical nitrosomethylurea in different mice strains. As indicated by the NIH 3T3 focus forming assay, a significant percentage of the tumors contain activated oncogenes of the ras family (K or N). Cloning and sequencing has enabled us to identify single base mutations as the only significant alteration present in the activated oncogenes. These alterations result in the substitution of amino-acid 12 or 61 of the p21 product of the ras genes. With the use of synthetic oligonucleotides it has been found that the tumors do not all contain the same mutation and in one case so far the normal allele is absent.
在不同品系的小鼠中，胸腺淋巴瘤可由γ射线辐射和化学物质亚硝基甲基脲诱导产生。如NIH 3T3焦点形成试验所示，相当比例的肿瘤含有ras家族（K或N）的活化癌基因。克隆和测序使我们能够确定单碱基突变是活化癌基因中唯一存在的显著改变。这些改变导致ras基因p21产物的第12或61位氨基酸被替换。使用合成寡核苷酸发现，肿瘤并非都含有相同的突变，到目前为止，在一个病例中正常等位基因缺失。
