Guerrero I, Calzada P, Mayer A, Pellicer A
Proc Natl Acad Sci U S A. 1984 Jan;81(1):202-5. doi: 10.1073/pnas.81.1.202.
By inducing mouse thymomas with carcinogens and gamma-radiation, we have studied the potential of tumor DNA to induce foci in rodent fibroblasts. A high percentage of the tumors used transformed the cultured cells, and the oncogenic phenotype segregated with extra copies of the c-ras gene family. There appears to be selectivity in the activated gene because so far all analyzed tumors induced by carcinogen have activated the N-ras gene, and those induced by radiation have activated the K-ras gene. The K-ras gene is the cellular counterpart of the viral ras oncogene in Kirsten murine sarcoma virus, but the N-ras has not yet been found in a retrovirus. The transformed cells have a marked increase in expression of the oncogene at the RNA and protein level. This model system might be a powerful tool in the study of leukemogenesis.
通过用致癌物和γ射线诱导小鼠胸腺瘤，我们研究了肿瘤DNA在啮齿动物成纤维细胞中诱导病灶的潜力。所使用的高比例肿瘤使培养细胞发生转化，并且致癌表型与c-ras基因家族的额外拷贝一起分离。激活的基因似乎具有选择性，因为到目前为止，所有经致癌物诱导分析的肿瘤都激活了N-ras基因，而那些经辐射诱导的肿瘤则激活了K-ras基因。K-ras基因是柯斯顿鼠肉瘤病毒中病毒ras癌基因的细胞对应物，但在逆转录病毒中尚未发现N-ras。转化细胞在RNA和蛋白质水平上癌基因的表达显著增加。该模型系统可能是白血病发生研究中的一个强大工具。
