Hormati Ahmad, Mirtalebi Roknabadi Fateme S, Aghaali Mohammad, Shams Saeed
Gastroenterology and Hepatology Disease Research Center, Qom University of Medical Sciences, Qom, Iran.
Faculty of Basic Sciences, Department of Genetics, Islamic Azad University Qom Branch, Qom, Iran.
Ann Med Surg (Lond). 2023 May 18;85(6):2298-2303. doi: 10.1097/MS9.0000000000000816. eCollection 2023 Jun.
Interleukin 17 (IL-17) plays an important role in the inflammation of the gastric mucosa and, in severe cases, the development of gastric cancer. Thus, the authors aimed to evaluate the A7488G polymorphism in () patients.
A total of 86 adults (in two -positive and -negative groups) were included in the study. To identify the infection, rapid urease test and polymerase chain reaction (PCR) were performed. The gene was also evaluated as a bacterial virulence factor. PCR-restriction fragment length polymorphism was used to investigate the A7488G polymorphism in gastric biopsies using the enzyme.
96.5% of patients in both groups did not show any mutation and had AA genotype, and only three patients infected with -carrying strains had polymorphism in the A7488G gene, which included AG (one case) and GG (two cases) patterns. No significant relationship was found between these polymorphisms in the two groups of -positive and -negative patients, while, interestingly, a significant difference was observed between the polymorphisms and the presence of the gene.
This report is one of the first to demonstrate the association of A7488G polymorphism with infection and the presence of the gene. Although no significant association between polymorphism and infection was found in the population of this study, the patients with mutated genotypes were positive for the gene, which was statistically significant. Therefore, the possibility of the role of pathogenic strains in causing mutations in cytokine genes is more conceivable.
白细胞介素17（IL - 17）在胃黏膜炎症中起重要作用，在严重情况下，还与胃癌的发生有关。因此，作者旨在评估（）患者中的A7488G多态性。
本研究共纳入86名成年人（分为两个阳性组和阴性组）。为确定感染情况，进行了快速尿素酶试验和聚合酶链反应（PCR）。还将该基因评估为一种细菌毒力因子。采用PCR - 限制性片段长度多态性方法，使用酶对胃活检组织中的A7488G多态性进行研究。
两组中96.5%的患者未显示任何突变，具有AA基因型，只有3名感染携带菌株的患者在A7488G基因中有多态性，其中包括AG（1例）和GG（2例）模式。在两组阳性和阴性患者中，这些多态性之间未发现显著关系，而有趣的是，在多态性与该基因的存在之间观察到显著差异。
本报告是首批证明A7488G多态性与感染及该基因存在之间关联的报告之一。尽管在本研究人群中未发现多态性与感染之间存在显著关联，但基因型突变的患者该基因呈阳性，这具有统计学意义。因此，致病菌株在导致细胞因子基因突变中发挥作用的可能性更值得考虑。
