Biology Department, East Tehran Branch, Islamic Azad University, Tehran, Iran.
Skin Research Centre, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Genes Immun. 2020 May;21(3):203-210. doi: 10.1038/s41435-020-0100-1. Epub 2020 May 1.
Alopecia areata is an autoimmune disease in which activation of autoreactive T cells and inflammatory immune signals target the hair follicles autoantigens. Although cytokines are involved in regulating autoimmune inflammation, the specific involvement of these molecules in the pathogenesis of alopecia areata has been remained unsettled. Here, a possible influence of IL12B, IL17A, and IL23R variations on susceptibility to alopecia areata in Iranian patients was investigated. Genotyping of IL12B (rs3212227), IL17A (rs2275913), and IL23R (rs10889677) variants were performed by extracting genomic DNA from patients and controls. Gene expression was analyzed by real-time RT-PCR. The frequency of IL12B and IL23R gene polymorphisms is significantly higher in the patients than controls, while no significant difference was found for IL17A. Stratification of the patients with respect to age at disease onset indicated that CC genotype of IL12B (rs3212227) and AA genotype of IL23R (rs10889677) gene polymorphisms are significantly associated with late-onset alopecia areata disease. In contrast to IL17A and IL23R, IL12B gene expression levels elevated in patients to that of controls, but genotypes had no effect on levels of gene expression. Overall, our data confirmed that the IL12B and IL23R polymorphisms are associated with the risk to develop alopecia areata in our population.
斑秃是一种自身免疫性疾病，其中自身反应性 T 细胞和炎症免疫信号的激活靶向毛囊自身抗原。虽然细胞因子参与调节自身免疫炎症，但这些分子在斑秃发病机制中的具体参与仍未确定。在这里，研究了 IL12B、IL17A 和 IL23R 变异对伊朗患者斑秃易感性的可能影响。通过从患者和对照中提取基因组 DNA 来进行 IL12B(rs3212227)、IL17A(rs2275913)和 IL23R(rs10889677)变异的基因分型。通过实时 RT-PCR 分析基因表达。与对照组相比，患者中 IL12B 和 IL23R 基因多态性的频率显着升高，而 IL17A 则没有显着差异。根据疾病发病年龄对患者进行分层表明，IL12B(rs3212227)的 CC 基因型和 IL23R(rs10889677)的 AA 基因型与斑秃的迟发性发病显着相关。与 IL17A 和 IL23R 相反，患者的 IL12B 基因表达水平高于对照组，但基因型对基因表达水平没有影响。总体而言，我们的数据证实了 IL12B 和 IL23R 多态性与我们人群中斑秃发病风险相关。
