Kaniuk Ewa, Lechowska-Liszka Agnieszka, Gajek Marcin, Nikodem Anna, Ścisłowska-Czarnecka Anna, Rapacz-Kmita Alicja, Stodolak-Zych Ewa
AGH University of Science and Technology, Al. Mickiewicza 30, Krakow, Poland.
AWF Academy of Physical Education, Al. Jana Pawła II 24, Krakow, Poland.
Biomater Adv. 2023 Sep;152:213506. doi: 10.1016/j.bioadv.2023.213506. Epub 2023 Jun 8.
Electrospinning is an increasingly popular technique for obtaining scaffolds for skin regeneration. However, electrospun scaffolds may also have some disadvantages, as the densely packed fibers in the scaffold structure can limit the penetration of skin cells into the inner part of the material. Such a dense arrangement of fibers can cause the cells to treat the 3D material as 2D one, and thus cause them to accumulate only on the upper surface. In this study, bi-polymer scaffolds made of polylactide (PLA) and polyvinyl alcohol (PVA) electrospun in a sequential or a concurrent system were investigated in a different PLA:PVA ratio (2:1 and 1:1). The properties of six types of model materials were investigated and compared i.e.; the initial materials electrospun by the sequential (PLA/PVA, 2PLA/PVA) and the concurrent system (PLA||PVA) and the same materials with removed PVA fibers (PLA/rPVA, 2PLA/rPVA, PLA||rPVA). The fiber models were intended to increase the porosity and coherent structure parameters of the scaffolds. The applied treatment involving the removal of PVA nanofibers increased the size of interfibrous pores formed between the PLA fibers. Ultimately, the porosity of the PLA/PVA scaffolds increased from 78 % to 99 %, and the time of water absorption decreased from 516 to 2 s. The change in wettability was induced by a synergistic effect of decrease in roughness after washing out and the presence of residual PVA fibers. The chemical analysis carried out confirmed the presence of PVA residues on the PLA fibers (FTIR-ATR study). In vitro studies were performed on human keratinocytes (HaKaT) and macrophages (RAW264.7), for which penetration into the inner part of the PLAIIPVA scaffold was observed. The new proposed approach, which allows the removal of PVA fibers from the bicomponent material, allows to obtain a scaffold with increased porosity, and thus better permeability for cells and nutrients.
静电纺丝是一种越来越受欢迎的用于获取皮肤再生支架的技术。然而，静电纺丝支架也可能存在一些缺点，因为支架结构中紧密排列的纤维会限制皮肤细胞渗透到材料内部。这种密集的纤维排列会使细胞将三维材料视为二维材料，从而导致它们仅在上表面积累。在本研究中，研究了由聚乳酸（PLA）和聚乙烯醇（PVA）在顺序或同时纺丝系统中静电纺丝制成的双聚合物支架，其PLA:PVA比例不同（2:1和1:1）。研究并比较了六种类型模型材料的性能，即：通过顺序（PLA/PVA、2PLA/PVA）和同时纺丝系统（PLA||PVA）静电纺丝的初始材料，以及去除PVA纤维后的相同材料（PLA/rPVA、2PLA/rPVA、PLA||rPVA）。纤维模型旨在增加支架的孔隙率和连贯结构参数。去除PVA纳米纤维的应用处理增加了PLA纤维之间形成的纤维间孔隙的尺寸。最终，PLA/PVA支架的孔隙率从78%增加到99%，吸水率从516秒降至2秒。润湿性的变化是由冲洗后粗糙度降低和残留PVA纤维的存在的协同效应引起的。进行的化学分析证实了PLA纤维上存在PVA残留物（傅里叶变换红外光谱衰减全反射研究）。对人角质形成细胞（HaKaT）和巨噬细胞（RAW264.7）进行了体外研究，观察到它们渗透到PLAIIPVA支架内部。新提出的方法允许从双组分材料中去除PVA纤维，从而获得具有更高孔隙率的支架，进而对细胞和营养物质具有更好的渗透性。
