Instituto de Macromoléculas Professora Eloisa Mano Universidade Federal do Rio de Janeiro (IMA/UFRJ), Brazil; Hybrid Systems Laboratory, Faculdade de Farmácia, Universidade Federal do Rio de Janeiro (UFRJ), Brazil.
Instituto de Macromoléculas Professora Eloisa Mano Universidade Federal do Rio de Janeiro (IMA/UFRJ), Brazil.
Mater Sci Eng C Mater Biol Appl. 2019 Apr;97:602-612. doi: 10.1016/j.msec.2018.12.020. Epub 2018 Dec 13.
Biocompatible scaffolds have been used to promote cellular growth and proliferation in order to develop grafts, prostheses, artificial skins and cartilage. Electrospinning is widely studied as a method capable of producing nanofibers which enables cell attachment and proliferation, generating a functional scaffold that is suitable for many types of organs or tissues. In this study, electrospinning was used to obtain core-shell and monolithic fibers from the biocompatible poly (lactic acid) and poly (vinyl alcohol) polymers. The main purpose of this work is to produce core-shell nanofiber based scaffolds that works as a sustained delivery vehicle for BMP-2 protein, allowing those fibers to be used in the recovery of alveolar bone tissue without further bone surgery. Then, polymer nanofibers were manufactured by optimizing process parameters of coaxial electrospinning with emphasis on the most relevant ones: voltage, internal and external flows in an attempt to correlate fibers properties with protein releasing abilities. All nanofibers were characterized according to its morphology, thermal behaviour, crystallinity and release profile. For the release tests, bovine albumin was added into internal fiber for future periodontal restorage application. Obtained results demonstrate that fibers were formed with diameters up to 250 nm. According to electronic microscopy images, one could observe surface of nanofibers, thickness and core-shell morphology confirmed. X-ray diffraction analysis and contact angle tests showed fibers with low crystal degree and low hydrophobicity. Nanofibers structure affected in vitro release model tests and consequently the cellular assays.
生物相容性支架已被用于促进细胞生长和增殖，以开发移植物、假体、人工皮肤和软骨。静电纺丝作为一种能够生产纳米纤维的方法被广泛研究，纳米纤维能够促进细胞附着和增殖，生成适合多种器官或组织的功能性支架。在这项研究中，使用静电纺丝从生物相容性的聚乳酸和聚乙烯醇聚合物中获得核壳和整体纤维。这项工作的主要目的是生产基于核壳纳米纤维的支架，作为 BMP-2 蛋白的持续释放载体，使这些纤维能够用于肺泡骨组织的恢复，而无需进一步的骨手术。然后，通过优化同轴静电纺丝的工艺参数来制造聚合物纳米纤维，重点是最相关的参数：电压、内外流量，试图将纤维的性质与蛋白质释放能力相关联。所有纳米纤维都根据其形态、热行为、结晶度和释放曲线进行了表征。对于释放测试，将牛血清白蛋白添加到内部纤维中，用于未来的牙周修复应用。获得的结果表明，纤维的直径可达 250nm。根据电子显微镜图像，可以观察到纳米纤维的表面、厚度和核壳形态得到了证实。X 射线衍射分析和接触角测试表明，纤维具有低结晶度和低疏水性。纳米纤维结构影响体外释放模型测试，进而影响细胞试验。
