Mahmood Arshad, Erum Alia, Tulain Ume Ruqia, Shafiq Sharmeen, Malik Nadia Shamshad, Khan Muhammad Tariq, Alqahtani Mohammed S
Faculty of Pharmacy, Al Ain University, Abu Dhabi Campus, Abu Dhabi P.O. Box 112612, United Arab Emirates.
AAU Health and Biomedical Research Center (HBRC), Al Ain University, Abu Dhabi P.O. Box 112612, United Arab Emirates.
Gels. 2023 Jun 8;9(6):474. doi: 10.3390/gels9060474.
The present study was conducted to fabricate and characterize mucilage-based polymeric networks of for controlled drug release. mucilage was used to develop a polymeric network via the free-radical polymerization method using potassium persulphate as the initiator, N' N'-Methylene bisacrylamide as the crosslinker, and acrylamide as the monomer. Using varying concentrations of mucilage, crosslinker, and monomer, we developed different formulations. Swelling studies were conducted at pH 1.2 and 7.4. Concentrations of polymer, monomer, and crosslinker were optimized as a function of swelling. Porosity and gel content were calculated for all samples. FTIR, SEM, XRD, TGA, and DSC studies were conducted for the characterization of polymeric networks. Thiocolchicoside was used as a model drug to study the in vitro release in acidic and alkaline pH. Various kinetics models were applied by using a DD solver. Increasing content of monomer and crosslinker swelling, porosity, and drug release decreased while gel content increased. An increase in mucilage concentration promotes swelling, porosity, and drug release of the polymeric network but decreases gel content. The FTIR study confirmed the formation of crosslinked networks. SEM indicated that the polymeric network had a porous structure. DSC and XRD studies indicated the entrapment of drugs inside the polymeric networks in amorphous form. The analytical method was validated according to ICH guidelines in terms of linearity, range, LOD, LOQ, accuracy, precision, and robustness. Analysis of drug release mechanism revealed Fickian behavior of all formulations. All these results indicated that the M1 formulation was considered to be the best polymeric network formulation in terms of sustaining drug release patterns.
本研究旨在制备并表征用于控释的基于黏液的聚合物网络。使用黏液通过自由基聚合法制备聚合物网络，以过硫酸钾为引发剂，N,N'-亚甲基双丙烯酰胺为交联剂，丙烯酰胺为单体。通过改变黏液、交联剂和单体的浓度，我们开发了不同的配方。在pH 1.2和7.4条件下进行溶胀研究。根据溶胀情况优化聚合物、单体和交联剂的浓度。计算所有样品的孔隙率和凝胶含量。进行傅里叶变换红外光谱（FTIR）、扫描电子显微镜（SEM）、X射线衍射（XRD）、热重分析（TGA）和差示扫描量热法（DSC）研究以表征聚合物网络。秋水仙碱硫代糖苷用作模型药物，研究其在酸性和碱性pH条件下的体外释放。使用DD求解器应用各种动力学模型。单体和交联剂含量增加时，溶胀、孔隙率和药物释放降低，而凝胶含量增加。黏液浓度增加会促进聚合物网络的溶胀、孔隙率和药物释放，但会降低凝胶含量。FTIR研究证实了交联网络的形成。SEM表明聚合物网络具有多孔结构。DSC和XRD研究表明药物以无定形形式包封在聚合物网络内部。根据国际协调会议（ICH）指南，对分析方法在线性、范围、检测限（LOD）、定量限（LOQ）、准确度、精密度和稳健性方面进行了验证。药物释放机制分析表明所有配方均呈现菲克行为。所有这些结果表明，就维持药物释放模式而言，M1配方被认为是最佳的聚合物网络配方。
