Yates-Stewart Ashley D, Yorke Benjamin T, Willse Alan, Fridley Jennifer, Head Graham P
Bayer Crop Science, Chesterfield, MO 63017, USA.
Insects. 2023 May 27;14(6):497. doi: 10.3390/insects14060497.
Transgenic crops are important tools for growers to manage insect pests, but their durability is threatened by the evolution of insect resistance. Implementing a resistance monitoring program is essential to detect and mitigate resistance. For non-high-dose crops, resistance monitoring is challenging, because insect control is not complete, so targeted insects and insect damage will be present even without resistance. Given these challenges, sentinel plots have been used to monitor for insect resistance to non-high-dose crops by assessing changes in the efficacy of a crop over time relative to a non- control. We optimized a sentinel plot resistance monitoring approach for MON 88702 ThryvOn™ cotton, a new non-high-dose product targeting two sucking pest taxa- ( and ) and thrips ( and )-and report here on the thrips monitoring methods and results. Quantifying thrips immatures was the best metric to characterize the impact of the trait, with at least a 40-60% average reduction of thrips immatures on ThryvOn relative to the control cotton at all sites with higher thrips densities. These data can be used within a ThryvOn resistance monitoring program and represent a case study for establishing a resistance monitoring approach for a non-high-dose trait product.
转基因作物是种植者防治害虫的重要工具，但其持久性受到昆虫抗性进化的威胁。实施抗性监测计划对于检测和减轻抗性至关重要。对于非高剂量作物，抗性监测具有挑战性，因为虫害防治并不彻底，所以即使没有抗性，目标害虫和虫害也会存在。鉴于这些挑战，已通过评估作物相对于非对照作物随时间的功效变化，利用哨兵地块来监测昆虫对非高剂量作物的抗性。我们针对MON 88702 ThryvOn™棉花优化了一种哨兵地块抗性监测方法，该棉花是一种针对两种刺吸式害虫类群（棉蚜和棉叶蝉）以及蓟马（西花蓟马和花蓟马）的新型非高剂量产品，并在此报告蓟马监测方法和结果。量化未成熟蓟马是表征该性状影响的最佳指标，在所有蓟马密度较高的地点，相对于对照棉花，ThryvOn上未成熟蓟马的平均减少率至少为40 - 60%。这些数据可用于ThryvOn抗性监测计划，并代表了为非高剂量性状产品建立抗性监测方法的一个案例研究。
