Akhtar Z R, Tian J C, Chen Y, Fang Q, Hu C, Peng Y F, Ye G Y
State Key Laboratory of Rice Biology, Key Laboratory of Agricultural Entomology, Ministry of Agriculture, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China.
Environ Entomol. 2013 Feb;42(1):173-80. doi: 10.1603/EN12055.
As a key component of ecological risk assessments, nontarget effects of Bacillus thuringiensis (Bt) rice have been tested under laboratory and field conditions for various organisms. A 2-yr field experiment was conducted to observe the nontarget effects of six transgenic rice lines (expressing the Cry1Ab or fused protein of Cry1Ab and Cry1Ac) on four nontarget thrips species including Frankliniella intonsa (Trybom), F. tenuicornis (Uzel), Haplothrips aculeatus (F.), and H. tritici (Kurd), as compared with their rice parental control lines. Two sampling methods including the beat plate and plastic bag method were used to monitor the population densities of the four thrips species for 2 yr. The results showed that the seasonal average densities of four tested thrips species in Bt rice plots were significantly lower than or very similar to those in the non-Bt rice plots depending on rice genotypes, sampling methods, and years. Among all six tested Bt rice lines, transgenic B1 and KMD2 lines suppressed the population of these tested thrips species the most. Our results indicate that the tested Bt rice lines are unlikely to result in high population pressure of thrips species in comparison with non-Bt rice. In some cases, Bt rice lines could significantly suppress thrips populations in the rice ecosystem. In addition, compatibility of Bt rice, with rice host plant resistance to nontarget sucking pests is also discussed within an overall integrated pest management program for rice.
作为生态风险评估的关键组成部分，苏云金芽孢杆菌（Bt）水稻对各种生物的非靶标效应已在实验室和田间条件下进行了测试。开展了一项为期两年的田间试验，以观察6种转基因水稻品系（表达Cry1Ab或Cry1Ab与Cry1Ac融合蛋白）对4种非靶标蓟马物种的非靶标效应，这4种蓟马分别是西花蓟马（Frankliniella intonsa (Trybom)）、烟蓟马（F. tenuicornis (Uzel)）、针蓟马（Haplothrips aculeatus (F.)）和麦蓟马（H. tritici (Kurd)），并将其与水稻亲本对照品系进行比较。采用拍打板法和塑料袋法两种采样方法，对这4种蓟马物种的种群密度进行了为期两年的监测。结果表明，根据水稻基因型、采样方法和年份，Bt水稻田4种受试蓟马物种的季节性平均密度显著低于或与非Bt水稻田非常相似。在所有6个受试Bt水稻品系中，转基因B1和KMD2品系对这些受试蓟马物种种群的抑制作用最大。我们的结果表明，与非Bt水稻相比，受试Bt水稻品系不太可能导致蓟马物种的高种群压力。在某些情况下，Bt水稻品系可显著抑制水稻生态系统中的蓟马种群。此外，还在水稻综合虫害管理总体方案的框架内讨论了Bt水稻与水稻寄主植物对非靶标刺吸式害虫抗性的兼容性。
