几丁质合酶基因在……的生长、应激反应和毒力方面有不同需求。

Chitin Synthase Genes Are Differentially Required for Growth, Stress Response, and Virulence in .

作者信息

Qin Jun, Zhao Peichen, Ye Ziqin, Sun Lifan, Hu Xiaoping, Zhang Jie

机构信息

State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Yangling 712100, China.

State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.

出版信息

J Fungi (Basel). 2022 Jun 28;8(7):681. doi: 10.3390/jof8070681.

Crop wilt disease caused by usually leads to serious yield loss. Chitin, an important component of most fungal cell walls, functions to maintain the rigidity of cell walls and septa. Chitin synthesis mainly relies on the activity of chitin synthase (CHS). Eight genes have been predicted in . In this study, we characterized the functions of these genes in terms of growth, stress responses, penetration, and virulence. Results showed that is important for conidia germination and resistance to hyperosmotic stress. Conidial production is significantly decreased in , , and mutants. , , , , , and genes are important for cell wall integrity, while all mutants are important for cell membrane integrity. All of the genes, except for , are required for the full pathogenicity of to and cotton plants. The in vitro and in vivo penetration of , , , and mutants was impaired, while that of the other mutants was normal. Overall, our results indicate that the genes exert diverse functions to regulate the growth and development, conidial germination, conidial production, stress response, penetration, and virulence in .

由……引起的作物枯萎病通常会导致严重的产量损失。几丁质是大多数真菌细胞壁的重要组成部分，其作用是维持细胞壁和隔膜的刚性。几丁质合成主要依赖于几丁质合酶（CHS）的活性。在……中已预测出八个……基因。在本研究中，我们从生长、应激反应、穿透和毒力方面对这些基因的功能进行了表征。结果表明，……对分生孢子萌发和耐高渗胁迫很重要。在……、……和……突变体中，分生孢子产量显著降低。……、……、……、……、……和……基因对细胞壁完整性很重要，而所有突变体对细胞膜完整性都很重要。除了……之外，所有……基因都是……对……和棉花植株具有完全致病性所必需的。……、……、……和……突变体的体外和体内穿透能力受损，而其他突变体的穿透能力正常。总体而言，我们的结果表明，……基因发挥多种功能来调节……的生长发育、分生孢子萌发、分生孢子产生、应激反应、穿透和毒力。