ESCET-Escuela Superior de Ciencias Experimentales y Tecnología, Departamento de Tecnología Química y Ambiental, Universidad Rey Juan Carlos, C/Tulipán s/n, Móstoles, 28933 Madrid, Spain.
CQM-Centro de Química da Madeira, Universidade da Madeira, Campus da Penteada, 9020-105 Funchal, Portugal.
Toxins (Basel). 2023 May 27;15(6):362. doi: 10.3390/toxins15060362.
Atropine and scopolamine belong to the tropane alkaloid (TA) family of natural toxins. They can contaminate teas and herbal teas and appear in infusions. Therefore, this study focused on analyzing atropine and scopolamine in 33 samples of tea and herbal tea infusions purchased in Spain and Portugal to determine the presence of these compounds in infusions brewed at 97 °C for 5 min. A rapid microextraction technique (µSPEed) followed by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was used to analyze the selected TAs. The results showed that 64% of the analyzed samples were contaminated by one or both toxins. White and green teas were generally more contaminated than black and other herbal teas. Of the 21 contaminated samples, 15 had concentrations above the maximum limit for liquid herbal infusions (0.2 ng/mL) set by Commission Regulation (EU) 2021/1408. In addition, the effects of heating conditions (time and temperature) on atropine and scopolamine standards and naturally contaminated samples of white, green, and black teas were evaluated. The results showed that at the concentrations studied (0.2 and 4 ng/mL), there was no degradation in the standard solutions. Brewing with boiling water (decoction) for 5 and 10 min allowed for higher extraction of TAs from dry tea to infusion water.
阿托品和东莨菪碱属于托烷生物碱（TA）天然毒素家族。它们可能会污染茶和花草茶，并出现在浸泡液中。因此，本研究专注于分析在西班牙和葡萄牙购买的 33 份茶和花草茶浸泡液中的阿托品和东莨菪碱，以确定在 97°C 下冲泡 5 分钟时这些化合物是否存在于浸泡液中。采用快速微萃取技术（µSPEed）结合高效液相色谱-串联质谱法（HPLC-MS/MS）来分析所选的托烷生物碱。结果表明，64%的分析样本受到一种或两种毒素的污染。白茶和绿茶通常比黑茶和其他花草茶污染更严重。在 21 个受污染的样本中，有 15 个样本的浓度超过了委员会法规（EU）2021/1408 规定的液体草药浸泡液的最大限量（0.2ng/mL）。此外，还评估了加热条件（时间和温度）对阿托品和东莨菪碱标准品以及白茶、绿茶和黑茶自然污染样本的影响。结果表明，在所研究的浓度（0.2 和 4ng/mL）下，标准溶液中没有降解。用沸水（煎煮）冲泡 5 分钟和 10 分钟可以从干茶中提取更多的托烷生物碱到浸泡液中。
