Department of Pathology, Centro Hospitalar de Vila Nova de Gaia/Espinho, Porto, Portugal.
Department of Pathology, Portuguese Oncology Institute of Porto (IPO Porto), Porto, Portugal.
Virchows Arch. 2023 Oct;483(4):549-554. doi: 10.1007/s00428-023-03578-7. Epub 2023 Jun 27.
Despite all precautions in pathology laboratories, contaminations and specimen mix-ups still occur and can negatively impact both patients and institutions. We present two cases in which short tandem repeat (STR) analysis was used to assert the correct identity of specimens. The first patient had a biopsy diagnosis of triple negative invasive carcinoma of no special type of the breast. Sample mix-up with another biopsy was suspected, because in her post-chemotherapy mastectomy specimen, a hormone receptor-positive lobular carcinoma was diagnosed. STR analysis displayed a complete common loci profile of the patient's biopsy and mastectomy, supporting that no mix-up occurred. The second patient underwent hysterectomy due to cervix squamous cell carcinoma. A fragment of adenocarcinoma was identified and confirmed by STR profile to be a contaminant. STR analysis is a fast, easy-to-perform, and widely available technique which can clarify contaminations and specimen mix-ups in pathology laboratories.
尽管在病理实验室采取了所有预防措施，但污染和标本混淆仍时有发生，可能会对患者和医疗机构产生负面影响。我们报告了两例使用短串联重复序列（STR）分析来确定标本正确身份的案例。第一例患者的乳腺活检诊断为三阴性浸润性非特殊型癌。怀疑与另一例活检标本发生了混淆，因为在她的化疗后乳房切除术标本中，诊断为激素受体阳性的小叶癌。STR 分析显示患者活检和乳房切除术标本的完全共同基因座图谱，支持未发生混淆。第二位患者因宫颈鳞状细胞癌接受了子宫切除术。鉴定出一段腺癌，并通过 STR 图谱证实为污染物。STR 分析是一种快速、易于操作且广泛可用的技术，可澄清病理实验室中的污染和标本混淆。
