Department of Neurology, Qing Dao Fu Wai Cardiovascular Hospital, Qingdao, 266000, Shandong, China.
Department of Neurology, Weifang People's Hospital, Weifang, 261000, Shandong, China.
BMC Neurol. 2023 Jun 27;23(1):248. doi: 10.1186/s12883-023-03241-3.
Acute cerebral infarction (ACI) is a common cerebrovascular disease. Previous studies have shown that some abnormally expressed microRNAs (miRNAs) play important roles in ACI. This study aimed to investigate the role of miR-106a-5p in the diagnosis and prognosis of ACI patients, and analyze the regulatory potential of miR-106a-5p on the inflammation of BV-2 microglial cells.
Serum and cerebrospinal fluid (CSF) samples were collected from 98 ACI patients, and the expression of serum miR-106a-5p was analyzed using qRT-PCR. A receiver operating characteristic (ROC) curve analysis was used to evaluate the diagnostic value of miR-106a-5p. The association of miR-106a-5p with ACI prognosis was evaluated using the logistic analysis. In vitro experiments were performed in BV-2 cells by oxygen glucose deprivation (OGD) treatment, and the effects of miR-106a-5p on BV-2 inflammation were assessed using an enzyme linked immunosorbent assay (ELISA).
It was observed that miR-106a-5p was significantly upregulated in the serum and CSF of ACI patients (all P < 0.001), and had considerable diagnostic accuracy. The highest serum miR-106a-5p was observed in severe ACI cases, and miR-106a-5p expression was significantly increased in unfavorable prognosis patients. Serum and CSF expression of miR-106a-5p was positively correlated with proinflammatory cytokines in ACI patients, and the inflammation of OGD-induced BV-2 cells was suppressed by miR-106a-5p reduction.
MiR-106a-5p is overexpressed in ACI patients and may serve as a diagnostic and prognostic biomarker for ACI. Furthermore, miR-106a-5p may be involved in ACI progression by regulating neuroinflammation.
急性脑梗死（ACI）是一种常见的脑血管病。既往研究表明，部分异常表达的 microRNAs（miRNAs）在 ACI 中发挥重要作用。本研究旨在探讨 miR-106a-5p 在 ACI 患者诊断和预后中的作用，并分析 miR-106a-5p 对 BV-2 小胶质细胞炎症的调控潜能。
收集 98 例 ACI 患者的血清和脑脊液（CSF）样本，采用 qRT-PCR 分析血清 miR-106a-5p 的表达。采用受试者工作特征（ROC）曲线分析评估 miR-106a-5p 的诊断价值。采用 logistic 分析评估 miR-106a-5p 与 ACI 预后的关系。通过氧葡萄糖剥夺（OGD）处理 BV-2 细胞进行体外实验，采用酶联免疫吸附试验（ELISA）评估 miR-106a-5p 对 BV-2 炎症的影响。
观察到 ACI 患者血清和 CSF 中 miR-106a-5p 显著上调（均 P<0.001），且具有相当的诊断准确性。严重 ACI 患者血清 miR-106a-5p 水平最高，预后不良患者 miR-106a-5p 表达显著增加。ACI 患者血清和 CSF 中 miR-106a-5p 的表达与促炎细胞因子呈正相关，miR-106a-5p 下调可抑制 OGD 诱导的 BV-2 细胞炎症。
miR-106a-5p 在 ACI 患者中过度表达，可能作为 ACI 的诊断和预后生物标志物。此外，miR-106a-5p 可能通过调节神经炎症参与 ACI 进展。
