National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
College of Animal Science, Tibet Agriculture and Animal Husbandry College, Linzhi 860000, China.
Cells. 2022 Jan 31;11(3):499. doi: 10.3390/cells11030499.
Subcutaneous fat thickness and intramuscular fat content are closely related to meat production and quality in the pig industry. Adipogenesis in adipocytes from subcutaneous and intramuscular fat tissues involves different genes and regulatory mechanisms. Analyzing the data of mRNA and miRNA transcriptomes during the differentiation of adipocytes from these two sources will help identify the different mechanisms of subcutaneous and intramuscular fat deposition. In this study, RNA sequencing technology was used to analyze the differential expression of genes and miRNAs in subcutaneous and intramuscular adipocytes at days 0, 2, 4, and 8 of differentiation. We mainly attributed the difference between fat depositions of the two types of adipocytes to variations in the expression patterns of related genes. Through combined weighted gene co-expression network analysis and K-MEANS, we identified 30 and 22 genes that mainly regulated the differentiation of subcutaneous adipocytes and intramuscular adipocytes, respectively. A total of 17 important candidate miRNAs were identified. This study provides valuable reference for the study of different mechanisms of adipogenesis among subcutaneous and intramuscular fat and contributes to improving pig breeding.
皮下脂肪厚度和肌内脂肪含量与猪产业的肉质和产量密切相关。皮下脂肪组织和肌内脂肪组织中的脂肪生成涉及不同的基因和调控机制。分析这两种来源的脂肪细胞分化过程中 mRNA 和 miRNA 转录组的数据，将有助于确定皮下脂肪和肌内脂肪沉积的不同机制。本研究采用 RNA 测序技术，分析了分化第 0、2、4 和 8 天的皮下和肌内脂肪细胞中基因和 miRNA 的差异表达。我们主要将两种脂肪细胞的脂肪沉积差异归因于相关基因表达模式的变化。通过联合加权基因共表达网络分析和 K-MEANS，我们分别鉴定出了 30 个和 22 个主要调控皮下脂肪细胞和肌内脂肪细胞分化的基因。共鉴定到 17 个重要的候选 miRNA。本研究为研究皮下脂肪和肌内脂肪的脂肪生成不同机制提供了有价值的参考，有助于改善猪的养殖。
