Gao Haihui, Liang Gaoxing, Su Na, Li Qirui, Wang Dong, Wang Jiandong, Zhao Long, Kang Xiaodong, Guo Kangkang
College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China.
Institute of Animal Science, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan 750002, China.
Animals (Basel). 2023 Jun 14;13(12):1983. doi: 10.3390/ani13121983.
spp., , and are significant parasitic gastrointestinal pathogens with global distribution in humans and domestic animals, including calves. The main symptoms of calf infection are severe diarrhea, dehydration, growth retardation, and sometimes even death. To date, there has been limited information on the prevalence of spp., , and infections in calves in Ningxia, China, especially between diarrheic and non-diarrheic calves. A total of 438 fecal samples were collected from diarrheic (201) and non-diarrheic (237) calves in Ningxia. PCR and DNA sequencing were used to find the prevalence of spp. at 46.8% (205/438), at 16.9% (74/438), and at 10.0% (44/438). The prevalence of spp. infection in diarrheic and non-diarrheic calves was 54.0% (128/237) and 38.3% (77/201), respectively, and statistical analysis showed a positive correlation between the prevalence of spp. infection and calf diarrhea ( < 0.01). However, in this study, there was no statistical correlation between the prevalence of infection as well as infection and calf diarrhea ( 0.05). Furthermore, four known species were successfully identified by comparing them with SSU rRNA gene sequences, including , , and . In addition, all 74 -positive samples were identified as assemblage E by comparative analysis of gene sequences. Among the 44 -positive samples sequenced in the present study, 4 distinct genotypes were successfully identified by comparative analysis of ITS sequences, including 3 known genotypes (J, BEB4, and N) and 1 novel genotype, the latter of which was identified and designated as NX1. These findings indicated that the high genetic diversity and complex population structures of spp., , and in Ningxia diarrhea calves and non-diarrhea calves, which provide new data for understanding the epidemiological status of spp., , and in Ningxia calves.
[某些寄生虫的名称]、[某些寄生虫的名称]和[某些寄生虫的名称]是重要的寄生性胃肠道病原体，在人类和包括犊牛在内的家畜中全球分布。犊牛感染的主要症状是严重腹泻、脱水、生长发育迟缓，有时甚至死亡。迄今为止，关于中国宁夏犊牛中[某些寄生虫的名称]、[某些寄生虫的名称]和[某些寄生虫的名称]感染率的信息有限，尤其是腹泻犊牛和非腹泻犊牛之间的情况。从宁夏腹泻（201头）和非腹泻（237头）犊牛中总共采集了438份粪便样本。采用聚合酶链反应（PCR）和DNA测序来检测[某些寄生虫的名称]的感染率为46.8%（205/438），[某些寄生虫的名称]为16.9%（74/438），[某些寄生虫的名称]为10.0%（44/438）。腹泻犊牛和非腹泻犊牛中[某些寄生虫的名称]感染率分别为54.0%（128/237）和38. (77/201)，统计分析表明[某些寄生虫的名称]感染率与犊牛腹泻之间存在正相关（P<0.01）。然而，在本研究中，[某些寄生虫的名称]感染率以及[某些寄生虫的名称]感染率与犊牛腹泻之间无统计学相关性（P>0.05）。此外，通过与小亚基核糖体RNA（SSU rRNA）基因序列比较，成功鉴定出4种已知的[某些寄生虫的名称]物种，包括[具体物种名称1]、[具体物种名称2]、[具体物种名称3]和[具体物种名称4]。另外，通过对[某些基因名称]基因序列的比较分析，所有74份[某些寄生虫的名称]阳性样本均被鉴定为E群。在本研究测序的44份[某些寄生虫的名称]阳性样本中，通过对内部转录间隔区（ITS）序列的比较分析，成功鉴定出4种不同的[某些寄生虫的名称]基因型，包括3种已知基因型（J、BEB4和N）和1种新基因型，后者被鉴定并命名为NX1。这些发现表明宁夏腹泻犊牛和非腹泻犊牛中[某些寄生虫的名称]属、[某些寄生虫的名称]属和[某些寄生虫的名称]属具有高度的遗传多样性和复杂的种群结构，为了解宁夏犊牛中[某些寄生虫的名称]属、[某些寄生虫的名称]属和[某些寄生虫的名称]属的流行病学状况提供了新的数据。
