Rucinque Daniel Santiago, van de Vis Hans, Reimert Henny, Roth Bjørn, Foss Atle, Taconeli Cesar Augusto, Gerritzen Marien
Faculdade de Zootecnia e Engenharia de Alimentos, Laboratório de Aquicultura, Universidade de São Paulo, Av. Duque de Caxias Norte 222, São Paulo 13635-900, Brazil.
Wageningen Livestock Research, P.O. Box 338, 6700 AH Wageningen, The Netherlands.
Animals (Basel). 2023 Jun 15;13(12):1993. doi: 10.3390/ani13121993.
As fish welfare becomes a growing concern, it is important to ensure humane treatment during slaughter. This study aimed to assess the onset of unconsciousness in Atlantic halibut immersed in CO-saturated seawater through electroencephalography (EEG). Of the 29 fish studied, 10 exhibited escape attempts, indicating aversion to CO-saturated water despite its oxygenation. EEG signals showed four distinct phases: transitional, excitation (high amplitude-high frequency), suppressed, and iso-electric phases. The onset of the suppressed phase, indicative of unconsciousness, occurred on average 258.8 ± 46.2 s after immersion. The spectral analysis of the EEG signals showed a progressive decrease in median frequency, spectral edge frequency, and high frequency contribution, which corresponded to the gradual loss of consciousness. The study concludes that CO-saturated water is not recommended for pre-slaughter handling of halibut due to the extended time required for the onset of unconsciousness and the observed aversive behaviour. Ensuring humane treatment during slaughter is important for addressing public concern and safeguarding fish welfare in all stages of production.
随着鱼类福利问题日益受到关注，确保屠宰过程中的人道对待至关重要。本研究旨在通过脑电图（EEG）评估浸泡在二氧化碳饱和海水中的大西洋大比目鱼失去意识的起始情况。在研究的29条鱼中，有10条表现出逃避行为，这表明尽管二氧化碳饱和海水已充氧，但它们仍厌恶这种环境。脑电图信号显示出四个不同阶段：过渡阶段、兴奋阶段（高振幅-高频）、抑制阶段和等电位阶段。表明失去意识的抑制阶段平均在浸泡后258.8±46.2秒出现。脑电图信号的频谱分析显示，中位数频率、谱边缘频率和高频成分逐渐下降，这与意识的逐渐丧失相对应。研究得出结论，由于失去意识所需时间较长以及观察到的厌恶行为，不建议将二氧化碳饱和海水用于大比目鱼的屠宰前处理。确保屠宰过程中的人道对待对于解决公众关切以及保障生产各阶段的鱼类福利至关重要。
