春大麦西伯利亚收集品中刺突产量性状的遗传剖析。

Genetic Dissection of Spike Productivity Traits in the Siberian Collection of Spring Barley.

机构信息

N.I. Vavilov All-Russian Research Institute of Plant Genetic Resources (VIR), 190000 St. Petersburg, Russia.

Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Lavrentjeva Ave. 10, 630090 Novosibirsk, Russia.

出版信息

Biomolecules. 2023 May 30;13(6):909. doi: 10.3390/biom13060909.

DOI:10.3390/biom13060909

PMID:37371489

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10295979/

Abstract

Barley ( L.) is one of the most commonly cultivated cereals worldwide. Its local varieties can represent a valuable source of unique genetic variants useful for crop improvement. The aim of this study was to reveal loci contributing to spike productivity traits in Siberian spring barley and to develop diagnostic DNA markers for marker-assisted breeding programs. For this purpose we conducted a genome-wide association study using a panel of 94 barley varieties. In total, 64 SNPs significantly associated with productivity traits were revealed. Twenty-three SNP markers were validated by genotyping in an independent sample set using competitive allele-specific PCR (KASP). Finally, fourteen markers associated with spike productivity traits on chromosomes 2H, 4H and 5H can be suggested for use in breeding programs.

摘要

大麦（Hordeum vulgare L.）是世界范围内广泛种植的谷物之一。其地方品种可能是独特遗传变异的宝贵来源，这些变异可用于作物改良。本研究旨在揭示影响西伯利亚春大麦穗产量性状的基因座，并开发用于标记辅助育种计划的诊断 DNA 标记。为此，我们使用 94 个大麦品种的小组进行了全基因组关联研究。总共发现了 64 个与生产力性状显著相关的 SNP。使用竞争性等位基因特异性 PCR（KASP）在独立样本集中对 23 个 SNP 标记进行了基因分型验证。最后，可以建议在 2H、4H 和 5H 染色体上与穗产量性状相关的 14 个标记用于育种计划。