Fang Yunxia, Zhang Xiaoqin, Zhang Xian, Tong Tao, Zhang Ziling, Wu Gengwei, Hou Linlin, Zheng Junjun, Niu Chunyu, Li Jia, Wang Wenjia, Wang Hua, Xue Dawei
College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China.
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
Front Plant Sci. 2020 Dec 17;11:620922. doi: 10.3389/fpls.2020.620922. eCollection 2020.
Grain size is an important agronomic trait determines yield in barley, and a high-density genetic map is helpful to accurately detect quantitative trait loci (QTLs) related to grain traits. Using specific-locus amplified fragment sequencing (SLAF-seq) technology, a high-density genetic map was constructed with a population of 134 recombinant inbred lines (RILs) deriving from a cross between Golden Promise (GP) and H602, which contained 12,635 SLAFs with 26,693 SNPs, and spanned 896.74 cM with an average interval of 0.07 cM on seven chromosomes. Based on the map, a total of 16 QTLs for grain length (GL), grain width and thousand-grain weight were detected on 1H, 2H, 4H, 5H, and 6H. Among them, a major QTL locus , accounting for the max phenotypic variance of 16.7% was located on 1H, which is a new unreported QTL affecting GL. In addition, the other two QTLs, and , accounting for the max phenotypic variances of 20.7 and 21.1%, respectively, were identified in the same region, and sequencing results showed they are identical to gene. These results indicate that it is a feasible approach to construct a high-quality genetic map for QTL mapping by using SLAF markers, and the detected major QTLs , , and are useful for marker-assisted selection (MAS) of grain size in barley breeding.
粒重是决定大麦产量的重要农艺性状，高密度遗传图谱有助于准确检测与粒重性状相关的数量性状位点（QTL）。利用特异性位点扩增片段测序（SLAF-seq）技术，以Golden Promise（GP）和H602杂交产生的134个重组自交系（RIL）群体构建了高密度遗传图谱，该图谱包含12,635个SLAF标记和26,693个单核苷酸多态性（SNP），覆盖7条染色体，全长896.74厘摩（cM），平均间隔0.07 cM。基于该图谱，在1H、2H、4H、5H和6H染色体上共检测到16个与粒长（GL）、粒宽和千粒重相关的QTL。其中，一个主要QTL位点位于1H染色体上，解释了高达16.7%的表型变异，这是一个影响粒长的新的未报道的QTL。此外，另外两个QTL，分别解释了20.7%和21.1%的最大表型变异，在同一区域被鉴定出来，测序结果表明它们与 基因相同。这些结果表明，利用SLAF标记构建高质量遗传图谱进行QTL定位是一种可行的方法，检测到的主要QTL 、 和 对大麦育种中粒重的分子标记辅助选择（MAS）具有重要意义。
