富含半胱氨酸的受体样蛋白激酶 CaCKR5 调节辣椒对青枯雷尔氏菌感染的免疫反应。

A cysteine-rich receptor-like protein kinase CaCKR5 modulates immune response against Ralstonia solanacearum infection in pepper.

机构信息

College of Life Science, Fujian Agriculture and Forestry University, Fujian, 350002, Fuzhou, People's Republic of China.

Key Laboratory of Applied Genetics of Universities in Fujian Province, Fujian Agriculture and Forestry University, Fujian, 350002, Fuzhou, People's Republic of China.

出版信息

BMC Plant Biol. 2021 Aug 19;21(1):382. doi: 10.1186/s12870-021-03150-y.

BACKGROUND

Cysteine-rich receptor-like kinases (CRKs) represent a large subfamily of receptor-like kinases and play vital roles in diverse physiological processes in regulating plant growth and development.

RESULTS

CaCRK5 transcripts were induced in pepper upon the infection of Ralstonia solanacearum and treatment with salicylic acid. The fusions between CaCRK5 and green fluorescence protein were targeted to the plasma membrane. Suppression of CaCRK5 via virus-induced gene silencing (VIGS) made pepper plants significantly susceptible to R. solanacearum infection, which was accompanied with decreased expression of defense related genes CaPR1, CaSAR8.2, CaDEF1 and CaACO1. Overexpression of CaCRK5 increased resistance against R. solanacearum in Nicotiana benthamiana. Furthermore, electrophoretic mobility shift assay and chromatin immunoprecipitation coupled with quantitative real-time PCR analysis revealed that a homeodomain zipper I protein CaHDZ27 can active the expression of CaCRK5 through directly binding to its promoter. Yeast two-hybrid and bimolecular fluorescence complementation (BiFC) analyses suggested that CaCRK5 heterodimerized with the homologous member CaCRK6 on the plasma membrane.

CONCLUSIONS

Our data revealed that CaCRK5 played a positive role in regulating immune responses against R. solanacearum infection in pepper.

背景

富含半胱氨酸的受体样激酶（CRKs）是受体样激酶大家族中的一个亚家族，在调节植物生长和发育的各种生理过程中发挥着重要作用。

结果

在辣椒受到青枯雷尔氏菌感染和水杨酸处理后，CaCRK5 的转录本被诱导。CaCRK5 与绿色荧光蛋白的融合蛋白被靶向到质膜。通过病毒诱导的基因沉默（VIGS）抑制 CaCRK5 会使辣椒植株对青枯雷尔氏菌感染的敏感性显著增加，同时防御相关基因 CaPR1、CaSAR8.2、CaDEF1 和 CaACO1 的表达水平降低。过表达 CaCRK5 可提高烟草原生质体对青枯雷尔氏菌的抗性。此外，电泳迁移率变动分析和染色质免疫沉淀结合定量实时 PCR 分析表明，同源盒结构域拉链 I 蛋白 CaHDZ27 可以通过直接结合其启动子来激活 CaCRK5 的表达。酵母双杂交和双分子荧光互补（BiFC）分析表明，CaCRK5 在质膜上与同源成员 CaCRK6 异源二聚化。