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在格拉夫氏髓性肿瘤模型中对来自[具体物种1]、[具体物种2]和[具体物种3]的血蓝蛋白的[具体物质1]和[具体物质2]抗肿瘤活性的评估。

Assessment of the and Antitumor Activity of Hemocyanins from , , and in a Graffi Myeloid Tumor Model.

作者信息

Georgieva Ani, Todorova Katerina, Iliev Ivan, Dilcheva Valeria, Vladov Ivelin, Petkova Svetlozara, Dolashki Aleksandar, Velkova Lyudmila, Dolashka Pavlina, Toshkova Reneta

机构信息

Institute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bl. 25, 1113 Sofia, Bulgaria.

Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bl. 9, 1113 Sofia, Bulgaria.

出版信息

Biomedicines. 2023 May 26;11(6):1545. doi: 10.3390/biomedicines11061545.

DOI:10.3390/biomedicines11061545
PMID:37371641
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10295749/
Abstract

Hemocyanins are oxygen-transporting glycoproteins in the hemolymph of some invertebrate species that attracted scientific interest as potential anticancer agents. The present study aims to assess the and anticancer activity of hemocyanins isolated from , , and in the Graffi myeloid tumor model. The antitumor activity of the hemocyanins was determined by a MTT test and cytomorphological analysis by fluorescent and transmission electron microscopy. The effects of the hemocyanins were examined in hamsters transplanted with Graffi tumor. The serum antibody titers against the tested hemocyanins and tumor antigen were determined by ELISA. Histopathological assessment of the morphological features related to antitumor effect, immune system response, and toxicity in some internal organs was performed. The results of studies indicated that the tested hemocyanins induced significant antiproliferative and apoptogenic effects. The investigations demonstrated a protective antitumor effect, expressed in reduced transplantability, suppression of tumor growth and metastasis, reduced mortality, prolonged survival time, and absence of toxic side effects. The present study indicated that the antitumor activity of the studied hemocyanins was due to both immune stimulation and direct effects on the tumor cells, and they displayed their potential as therapeutic agents against hematological malignances.

摘要

血蓝蛋白是一些无脊椎动物血淋巴中运输氧气的糖蛋白，作为潜在的抗癌剂引起了科学界的兴趣。本研究旨在评估从[具体物种1]、[具体物种2]和[具体物种3]中分离出的血蓝蛋白在格拉菲髓样肿瘤模型中的抗癌活性。通过MTT试验以及荧光和透射电子显微镜进行细胞形态学分析来确定血蓝蛋白的抗肿瘤活性。在移植了格拉菲肿瘤的仓鼠中检测血蓝蛋白的效果。通过ELISA测定针对测试血蓝蛋白和肿瘤抗原的血清抗体滴度。对一些内部器官中与抗肿瘤作用、免疫系统反应和毒性相关的形态学特征进行组织病理学评估。[具体研究]的结果表明，测试的血蓝蛋白诱导了显著的抗增殖和凋亡作用。[具体调查]证明了一种保护性抗肿瘤作用，表现为移植性降低、肿瘤生长和转移受到抑制、死亡率降低、存活时间延长以及无毒性副作用。本研究表明，所研究的血蓝蛋白的抗肿瘤活性是由于免疫刺激和对肿瘤细胞的直接作用，并且它们展现出作为抗血液恶性肿瘤治疗剂的潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a588/10295749/6b4b88744cbf/biomedicines-11-01545-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a588/10295749/5b5bfd11922a/biomedicines-11-01545-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a588/10295749/b8f8c612b9ab/biomedicines-11-01545-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a588/10295749/104d39c93748/biomedicines-11-01545-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a588/10295749/3310534a5217/biomedicines-11-01545-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a588/10295749/424152e28b2a/biomedicines-11-01545-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a588/10295749/969853ed48e8/biomedicines-11-01545-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a588/10295749/025967f70c33/biomedicines-11-01545-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a588/10295749/eab3f30f5069/biomedicines-11-01545-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a588/10295749/6b4b88744cbf/biomedicines-11-01545-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a588/10295749/5b5bfd11922a/biomedicines-11-01545-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a588/10295749/b8f8c612b9ab/biomedicines-11-01545-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a588/10295749/104d39c93748/biomedicines-11-01545-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a588/10295749/3310534a5217/biomedicines-11-01545-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a588/10295749/424152e28b2a/biomedicines-11-01545-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a588/10295749/969853ed48e8/biomedicines-11-01545-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a588/10295749/025967f70c33/biomedicines-11-01545-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a588/10295749/eab3f30f5069/biomedicines-11-01545-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a588/10295749/6b4b88744cbf/biomedicines-11-01545-g009.jpg

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