Faculty of Chemistry, Warsaw University of Technology, Noakowskiego 3 Street, 00-664 Warsaw, Poland.
The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Instytucka 3 Street, 05-110 Jabłonna, Poland.
Int J Mol Sci. 2024 Sep 15;25(18):9958. doi: 10.3390/ijms25189958.
In recent years, snail mucus (SM) has become popular as an active ingredient in cosmetic and pharmaceutical products. In turn, snail eggs (SEs) also seem to be a promising active compound, but the biological activities of SEs are significantly less known. Therefore, our preliminary study aimed to compare the biological activities of the SEs and SM of Müller. The metabolomic analysis (LC-MS technique), determination of the antimicrobial activity (agar diffusion test, broth microdilution methods), antioxidant activity (ABTS assay), cytotoxicity assay (MTT), and proapoptotic properties (flow cytometry) of the SEs and SM were evaluated. It was found that the SEs and SM contain 8005 and 7837 compounds, respectively. The SEs showed antibacterial activity against (MIC 12.5 mg/mL) and (MIC 3.12 mg/mL). The EC50 estimation of the antioxidant activity is 89.64 mg/mL and above 100 mg/mL for the SEs and SM, respectively. The SEs also inhibited the cell proliferation of cancer cell lines (HCT-116, MCF-7, HT-29) more strongly compared to the SM. The highest proportion of apoptotic cells in HCT-116 was observed. The reach composition of the compounds in the SEs and SM may be crucial for the creation of new cosmetic and pharmaceutical raw materials with different biological activities. However, further extended studies on the biological activities of -delivered materials are still necessary.
近年来，蜗牛黏液 (SM) 已成为化妆品和制药产品中一种流行的活性成分。反过来，蜗牛卵 (SEs) 似乎也是一种很有前途的活性化合物，但 SEs 的生物活性知之甚少。因此，我们的初步研究旨在比较 Müller 的 SEs 和 SM 的生物活性。采用代谢组学分析 (LC-MS 技术)、抗菌活性测定 (琼脂扩散试验、肉汤微量稀释法)、抗氧化活性测定 (ABTS 测定法)、细胞毒性测定 (MTT 法) 和促凋亡特性 (流式细胞术) 对 SEs 和 SM 进行了评估。结果发现，SEs 和 SM 分别含有 8005 种和 7837 种化合物。SEs 对 和 （MIC12.5mg/mL）具有抗菌活性。抗氧化活性的 EC50 估计值分别为 SEs 和 SM 的 89.64mg/mL 以上和 100mg/mL 以上。与 SM 相比，SEs 还能更强烈地抑制癌细胞系（HCT-116、MCF-7、HT-29）的细胞增殖。在 HCT-116 中观察到凋亡细胞的比例最高。SEs 和 SM 中化合物的组成可能对创造具有不同生物活性的新型化妆品和制药原料至关重要。然而，仍有必要对 -delivered 材料的生物活性进行进一步的扩展研究。
