Bai Xuefei, Li Qian, Zhang Dai, Zhao Yi, Zhao Dongmei, Pan Yang, Wang Jinhui, Yang Zhihui, Zhu Jiehua
College of Plant Protection, Hebei Agricultural University, Baoding 071000, China.
Biology (Basel). 2023 Jun 14;12(6):856. doi: 10.3390/biology12060856.
HN-Q-8, isolated in our previous study, has an antagonistic effect on . After being pretreated with a fermentation liquid with HN-Q-8 bacterial cell suspensions, the potato leaves inoculated with displayed smaller lesion areas and less yellowing than the controls. Interestingly, the activity levels of superoxide dismutase, peroxidase, and catalase in potato seedlings were enhanced by the addition of the fermentation liquid with bacterial cells. Additionally, the overexpression of key genes related to induced resistance in the Jasmonate/Ethylene pathway was activated by the addition of the fermentation liquid, suggesting that the HN-Q-8 strain induced resistance to potato early blight. In addition, our laboratory and field experiments showed that the HN-Q-8 strain can promote potato seedling growth and significantly increase tuber yield. The root activity and chlorophyll content of potato seedlings were significantly increased along with the levels of indole acetic acid, gibberellic acid 3, and abscisic acid upon addition of the HN-Q-8 strain. The fermentation liquid with bacterial cells was more efficient in inducing disease resistance and promoting growth than bacterial cell suspensions alone or the fermentation liquid without bacterial cells. Thus, the HN-Q-8 strain is an effective bacterial biocontrol agent, augmenting the options available for potato cultivation.
我们之前的研究中分离出的HN-Q-8对……具有拮抗作用。用含有HN-Q-8细菌细胞悬液的发酵液预处理后，接种……的马铃薯叶片与对照相比，病斑面积更小，黄化程度更低。有趣的是，添加含有细菌细胞的发酵液可提高马铃薯幼苗中超氧化物歧化酶、过氧化物酶和过氧化氢酶的活性水平。此外，添加发酵液可激活茉莉酸/乙烯途径中与诱导抗性相关的关键基因的过表达，这表明HN-Q-8菌株可诱导马铃薯对早疫病的抗性。此外，我们的实验室和田间试验表明，HN-Q-8菌株可促进马铃薯幼苗生长并显著提高块茎产量。添加HN-Q-8菌株后，马铃薯幼苗的根系活力和叶绿素含量以及吲哚乙酸、赤霉酸3和脱落酸的水平均显著增加。含有细菌细胞的发酵液在诱导抗病性和促进生长方面比单独的细菌细胞悬液或不含细菌细胞的发酵液更有效。因此，HN-Q-8菌株是一种有效的细菌生物防治剂，增加了马铃薯种植的可用选择。
