Comparative Analysis of Water-Soluble Polysaccharides from Second Love 'Tokimeki' and in Structure, Antioxidant, and Anti-Tumor Activity In Vitro.

With potential anti-tumor and antioxidant properties, the polysaccharide content of is relatively lower than that of the other medicinal To find high-content polysaccharide resources, the polysaccharide (DHPP-Ⅰs) was prepared from Second Love 'Tokimeki' (a hybrid) and compared with DNPP-Ⅰs from . DHPP-Is (Mn 31.09 kDa) and DNPP-Is (Mn 46.65 kDa) were found to be -acetylated glucomannans (-Glc-(1,4) and -acetylated-D-Man-(1,4) backbones), analogous to other polysaccharides. DHPP-Ⅰs had higher glucose content (31.1%) and a lower degree (0.16) of acetylation than DNPP-Ⅰs (15.8%, 0.28). Meanwhile, DHPP-Ⅰs and DNPP-Ⅰs had the same ability in the radical scavenging assay, which was milder than the control of Vc. Both DHPP-Is and DNPP-Is inhibited cell proliferation in vitro, with obvious differences in dose concentrations (0.5-2.0 mg/mL) and treatment times (24-72 h). Therefore, the antioxidant activity of DHPP-Ⅰs and DNPP-Ⅰs is not associated with distinction in anti-proliferative activity. As a glucomannan derived from non-medicinal , DHPP-Ⅰs has similar bioactivity to other medicinal and this could serve as a starting point for studying the conformational-bioactivity relationship of polysaccharides.