Sánchez-Trujillo Lara, Fraile-Martinez Oscar, García-Montero Cielo, García-Puente Luis M, Guijarro Luis G, De Leon-Oliva Diego, Boaru Diego Liviu, Gardón-Alburquerque David, Del Val Toledo Lobo María, Royuela Mar, García-Tuñón Ignacio, Rios-Parra Antonio, De León-Luis Juan A, Bravo Coral, Álvarez-Mon Melchor, Bujan Julia, Saez Miguel A, García-Honduvilla Natalio, Ortega Miguel A
Department of Medicine and Medical Specialities, Faculty of Medicine and Health Sciences, University of Alcalá, 28801 Alcalá de Henares, Spain.
Ramón y Cajal Institute of Sanitary Research (IRYCIS), 28034 Madrid, Spain.
J Pers Med. 2023 Jun 5;13(6):956. doi: 10.3390/jpm13060956.
Chronic venous disease (CVD) is a common condition that affects the veins in the lower limbs, resulting in a variety of symptoms, such as swelling, pain, and varicose veins (VVs). The plenty hormonal, hemodynamic and mechanical changes occurred in pregnancy make women especially vulnerable to suffer from this condition in this period. Previous works have identified that CVD is associated with an increased inflammatory milieu and significant damage in maternofetal tissues, such as the umbilical cord. However, the inflammatory status of this structure in these patients has not been studied yet. Thus, the aim of the present study was to examine gene and protein expression of a set of inflammatory markers-Allograft inflammatory factor 1 (AIF-1), the proinflammatory cytokines interleukin 12A (IL-12A) and IL-18 and the anti-inflammatory product IL-10-in the umbilical cord of women with CVD during pregnancy (N = 62) and healthy pregnant women (HC; N = 52) by the use of real time qPCR and immunohistochemistry (IHC). Our results demonstrate that the umbilical cord tissue from CVD women exhibit an increased expression of AIF-1, IL-12A and IL-18 along with a decrease in IL-10. Therefore, our study suggests an inflammatory status of this structure related to CVD. Further studies should be conducted to evaluate the expression of other inflammatory markers, as well as to analyze the maternofetal impact of these findings.
慢性静脉疾病（CVD）是一种常见病症，会影响下肢静脉，导致多种症状，如肿胀、疼痛和静脉曲张（VV）。孕期发生的大量激素、血流动力学和机械变化使女性在此期间特别容易患这种疾病。先前的研究已经确定，CVD与炎症环境增加以及母婴组织（如脐带）的显著损伤有关。然而，这些患者中该结构的炎症状态尚未得到研究。因此，本研究的目的是通过实时定量聚合酶链反应（qPCR）和免疫组织化学（IHC）检测一组炎症标志物——同种异体移植炎症因子1（AIF-1）、促炎细胞因子白细胞介素12A（IL-12A）和IL-18以及抗炎产物IL-10在孕期患有CVD的女性（N = 62）和健康孕妇（HC；N = 52）脐带中的基因和蛋白表达。我们的结果表明，患有CVD的女性的脐带组织中AIF-1、IL-12A和IL-18的表达增加，而IL-10的表达减少。因此，我们的研究表明该结构的炎症状态与CVD有关。应进行进一步研究以评估其他炎症标志物的表达，并分析这些发现对母婴的影响。
