Nallaseth F S, Dewey M J
Nucleic Acids Res. 1986 Jul 11;14(13):5295-307. doi: 10.1093/nar/14.13.5295.
Male specific repeated sequences compose approximately 10% of the mouse Y chromosome. This was deduced from studies of genomic phage clones that contain male specific DNA, and three subcloned EcoRI fragments pBC10-0.6, pBC15-1.1, and pBA33-1.8. Southern analyses and in situ hybridization of metaphase chromosomes show these three sequences to be present in 100-200 copies on the Y chromosome and in female DNA as single copies. One of these, pBC10-0.6, was directly demonstrated to be on the X chromosome. All three exhibited male specific transcription. These sequences are found in two distinct organizations. Subclone pBC10-0.6 is uniformly found in a long HpaI repeat unit of 9.7 Kbp. Subclones pBC15-1.1 and pBA33-1.8 are distributed throughout several EcoR1 repeat families in which the male specific fragments are interspersed. These male specific fragments also differ among themselves with regard to the types and frequency of evolutionary changes.
雄性特异性重复序列约占小鼠Y染色体的10%。这是通过对包含雄性特异性DNA的基因组噬菌体克隆以及三个亚克隆的EcoRI片段pBC10 - 0.6、pBC15 - 1.1和pBA33 - 1.8的研究推断出来的。中期染色体的Southern分析和原位杂交显示，这三个序列在Y染色体上以100 - 200个拷贝存在，而在雌性DNA中为单拷贝。其中一个，pBC10 - 0.6，被直接证明位于X染色体上。所有三个序列都表现出雄性特异性转录。这些序列存在于两种不同的组织形式中。亚克隆pBC10 - 0.6均匀地存在于一个9.7 Kbp的长HpaI重复单元中。亚克隆pBC15 - 1.1和pBA33 - 1.8分布在几个EcoR1重复家族中，其中雄性特异性片段是散布的。这些雄性特异性片段在进化变化的类型和频率方面也彼此不同。
