Departement of Medical Physiology, Faculty of Medicine, Universitas Muhammadiyah Prof. Dr. Hamka, Jakarta 121130, Indonesia.
Graduate Student in Doctoral Program in Medical Sciences, Faculty of Medicine Universitas Indonesia, Jakarta 10430, Indonesia.
Medicina (Kaunas). 2023 May 28;59(6):1044. doi: 10.3390/medicina59061044.
: Non-alcoholic Fatty Liver Disease (NAFLD) can occur as a result of micronutrient deficiencies. Hibiscus sabdarifa, a plant used in traditional medicine, contains ingredients that can help prevent this process. This study looked at the potency of Ethanol Extract (HSE) to prevent homocysteine-induced liver damage in animals that were deficient in vitamin B12. : A comparative study of the effects of roselle extract is presented in an experimental design. Thirty Sprague-Dawley rats were divided into six groups using randomization. To demonstrate the absence of liver damage in the experimental animals under normal conditions, a control group was fed a normal diet without HSE. For the induction of liver damage in the experimental animals, the vitamin B12-restricted group was administered a vitamin B12-restricted diet. To test the effect of HSE on liver damage, the treatment group was given HSE along with a vitamin B12-restricted diet. Each group was given two treatment periods of eight and sixteen weeks. These results were compared with the results of the parameter examination between the vitamin B12 restriction group, with and without HSE, using an ANOVA statistic. The data were analyzed with licensed SPSS 20.0 software. : HSE significantly increased the blood levels of vitamin B12 while lowering homocysteine levels. The administration of HSE reduced liver damage based on the activity of liver function enzymes in the plasma due to a limitation of vitamin B12. HSE decreased Sterol Regulatory Element-Binding Protein-1c (SREBP1c) and Nuclear Factor Kappa B (NFkB) protein expressions in the liver tissue, but did not decrease Glucose-Regulated Protein 78 (GRP78) protein expression. Significantly, the levels of Tumor Necrosis Factor alpha (TNF-a) and IL-6 in the liver tissue were lower, while the levels of IL-10 and Nuclear factor-erythroid-2 Related Factor 2 (NRF2) were higher with HSE administration. HSE produced a better histopathological profile of the Hematoxylin and Eosin (H&E)-Masson tricrome for inflammation, fat and fibrosis in the liver. : In this study, HSE was found to slow the development of liver damage in experimental animals that were given a vitamin B12-deficient diet.
非酒精性脂肪性肝病 (NAFLD) 可能是由于微量营养素缺乏引起的。在传统医学中使用的植物芙蓉 sabdarifa 含有可以帮助预防这种过程的成分。这项研究着眼于乙醇提取物 (HSE) 预防维生素 B12 缺乏动物中同型半胱氨酸诱导的肝损伤的效力。
在实验设计中提出了罗勒提取物的影响的比较研究。使用随机化将 30 只 Sprague-Dawley 大鼠分为六组。为了证明在正常条件下实验动物没有肝损伤，对照组给予不含 HSE 的正常饮食。为了诱导实验动物的肝损伤，维生素 B12 限制组给予维生素 B12 限制饮食。为了测试 HSE 对肝损伤的影响，治疗组给予 HSE 加维生素 B12 限制饮食。每组给予两个 8 周和 16 周的治疗期。使用 ANOVA 统计数据将这些结果与维生素 B12 限制组与 HSE 之间的参数检查结果进行比较。使用许可的 SPSS 20.0 软件分析数据。
HSE 显著增加了血液中维生素 B12 的水平，同时降低了同型半胱氨酸水平。由于维生素 B12 的限制，HSE 降低了血浆中肝功能酶的肝损伤活性。HSE 降低了肝脏组织中的固醇调节元件结合蛋白-1c (SREBP1c) 和核因子 kappa B (NFkB) 蛋白表达，但没有降低葡萄糖调节蛋白 78 (GRP78) 蛋白表达。重要的是，HSE 给药后，肝脏组织中的肿瘤坏死因子 alpha (TNF-a) 和 IL-6 水平降低，而 IL-10 和核因子-红细胞相关因子 2 (NRF2) 水平升高。HSE 对肝脏的苏木精和伊红 (H&E)-Masson 三色法炎症、脂肪和纤维化产生了更好的组织病理学特征。
在这项研究中，发现 HSE 可减缓给予维生素 B12 缺乏饮食的实验动物肝损伤的发展。
