Department of Physiology, Faculty of Medical Science, Naresuan University, 99 Moo 9, Tahpoh, Muang, Phitsanulok, 65000, Thailand.
Department of Pharmacy Practice, Faculty of Pharmaceutical Sciences, Naresuan University, Phitsanulok, 65000, Thailand.
Sci Rep. 2022 Apr 29;12(1):7022. doi: 10.1038/s41598-022-11027-9.
Non-alcoholic fatty liver disease (NAFLD) has become a major world-wide health problem and is characterized by lipid accumulation in the liver induced by high fat diet (HFD) consumption. It is usually associated with inflammation, oxidative stress, and insulin resistance. Roselle extract (Hibiscus sabdariffa) is an herb which is used in traditional medicine. However, further study is necessary to represent the mechanism of NAFLD and find new preventive strategies. This study aims to investigate the protective effects of roselle extract on NAFLD rat models. Male Sprague-Dawley rats (n = 35) were divided into 5 groups, control, HFD, HFD + Simvastatin (HFD + SIM), HFD + 250 mg/kg BW, and HFD + 500 mg/kg BW of roselle extract (HFD + R250 and HFD + R500, respectively). The results showed that roselle extract reduced hepatic lipid contents, de novo lipogenesis enzymes, microsomal triglyceride transfer protein, inflammatory cytokines, malondialdehyde, and increased antioxidant properties, transporter related with lipoprotein uptake, and insulin signal proteins. Comparing to SIM, the HFD + R500 group exhibited the greater benefit in terms of anti-hepatic steatosis, antioxidant properties, and an ability to improve insulin resistance. This study demonstrates that roselle extract improved antioxidant properties and attenuated hepatic steatosis, liver inflammation, oxidative stress, and insulin resistance in HFD-induced NAFLD in rats, which could be used for NAFLD prevention.
非酒精性脂肪性肝病（NAFLD）已成为全球性重大健康问题，其特征是高脂肪饮食（HFD）摄入引起的肝脏脂质积累。它通常与炎症、氧化应激和胰岛素抵抗有关。洛神花提取物（Hibiscus sabdariffa）是一种传统医学中使用的草药。然而，需要进一步的研究来代表 NAFLD 的机制并找到新的预防策略。本研究旨在探讨洛神花提取物对 NAFLD 大鼠模型的保护作用。雄性 Sprague-Dawley 大鼠（n=35）分为 5 组，对照组、HFD 组、HFD+辛伐他汀（HFD+SIM）组、HFD+250mg/kg BW 组和 HFD+500mg/kg BW 组的洛神花提取物（HFD+R250 和 HFD+R500）。结果表明，洛神花提取物降低了肝脂质含量、从头合成脂肪酶、微粒体甘油三酯转移蛋白、炎性细胞因子、丙二醛，并增加了抗氧化特性、与脂蛋白摄取相关的转运体和胰岛素信号蛋白。与 SIM 相比，HFD+R500 组在抗肝脂肪变性、抗氧化特性和改善胰岛素抵抗方面表现出更大的益处。本研究表明，洛神花提取物改善了抗氧化特性，并减轻了 HFD 诱导的 NAFLD 大鼠的肝脂肪变性、肝炎症、氧化应激和胰岛素抵抗，可用于预防 NAFLD。
