Paulenco Anca, Vintila Alin Cristian Nicolae, Vlaicu Alexandru, Ciltea-Udrescu Mihaela, Galan Ana-Maria
National Institute for Research & Development in Chemistry and Petrochemistry-ICECHIM, 202 Spl. Independentei, 060021 Bucharest, Romania.
Faculty of Chemical Engineering and Biotechnologies, University Politehnica of Bucharest, 1-7 Polizu Street, 011061 Bucharest, Romania.
Microorganisms. 2023 May 31;11(6):1469. doi: 10.3390/microorganisms11061469.
This paper focuses on a process for dairy wastewater treatment by mixotrophic cultivation of microalgae sp., using cheese whey obtained as a side flow from cheese production as an organic carbon source. The microalgae samples were prepared by adding to the standard growth medium increasing amounts of cheese whey, calculated to ensure a lactose concentration between 0 and 10 g/L. The samples were incubated at a constant temperature of 28 °C and 175 rpm stirring speed for a total time of seven days. Two LED (Light Emitting Diode) illumination schemes were applied in order to assess the effect of this parameter on microalgae development and bioactive compound accumulation: continuous illumination (light stress) versus alternative cycles of 12 h light-12 h dark (day-night cycle). The growth medium was analyzed before and after microalgae cultivation in order to determine the reduction of carbon, nitrogen, and phosphorus. The results obtained for this process, after a seven-day cultivation period, were as follows: reduction of 99-100% of lactose from the growth medium, up to 96% reduction in chemical oxygen demand, up to 91% reduction in nitrogen content, and up to 70% reduction in phosphorus content.
本文重点研究了利用从奶酪生产中作为副产品获得的奶酪乳清作为有机碳源，通过混合营养培养微藻来处理乳制品废水的工艺。微藻样品通过向标准生长培养基中添加逐渐增加量的奶酪乳清来制备，添加量经计算以确保乳糖浓度在0至10克/升之间。样品在28°C的恒定温度和175转/分钟的搅拌速度下孵育7天。应用了两种发光二极管（LED）照明方案，以评估该参数对微藻生长和生物活性化合物积累的影响：连续照明（光胁迫）与12小时光照 - 12小时黑暗的交替循环（昼夜循环）。在微藻培养前后对生长培养基进行分析，以确定碳、氮和磷的减少情况。经过7天的培养期，该工艺获得的结果如下：生长培养基中乳糖减少99 - 100%，化学需氧量减少高达96%，氮含量减少高达91%，磷含量减少高达70%。
