Shen Yunxin, Zhao Jiangyuan, Zou Xuefeng, Shi Zhufeng, Liao Yongqin, He Yonghong, Wang Hang, Chen Qibin, Yang Peiweng, Li Minggang
College of Plant Protection, Yunnan Agricultural University, Kunming 655508, China.
Institute of Agricultural Environment and Resources, Yunnan Academy of Agricultural Sciences, Kunming 650204, China.
Microorganisms. 2023 Jun 9;11(6):1535. doi: 10.3390/microorganisms11061535.
Siderophores secreted by microorganisms can promote ecological efficiency and could be used to regulate the unbalanced microbial community structure. The influence of the siderophore activity of Trichoderma yunnanense strain 2-14F2 and Beauveria pseudobassiana strain (2-8F2) on the physiological/biochemical functions and community structure of soil microbes affected by tobacco bacterial wilt (TBW) was studied. DNS Colorimetry and Biolog-eco plates were used to quantify the impacts of strain siderophores on soil enzyme activities and microbial metabolism. Based on Illumina MiSeq high-throughput sequencing, the soil 16S rDNA and ITS sequences were amplified to dissect the response characteristics of alpha/beta diversity and the structure/composition of a soil microbial community toward siderophores. The KEGG database was used to perform the PICRUSt functional prediction of the microbial community. We found that siderophores of 2-14F2 and 2-8F2, at certain concentrations, significantly increased the activities of sucrase (S-SC) and urease (S-UE) in the TBW soil and enhanced the average well color development (AWCD, carbon source utilization capacity) of the microbial community. The metabolic capacity of the diseased soil to amino acids, carbohydrates, polymers, aromatics, and carboxylic acids also increased significantly. The response of the bacterial community to siderophore active metabolites was more significant in alpha diversity, while the beta diversity of the fungal community responded more positively to siderophores. The relative abundance of , , and increased and was accompanied by reductions in and . LEfSe analysis showed that , , , and altered the most under different concentrations of siderophore active metabolites. The PICRUSt functional prediction results showed that siderophore increased the abundance of the redox-related enzymes of the microbial community in TBW soil. The BugBase phenotypic prediction results showed that the siderophore activity could decrease the abundance of pathogenic bacteria. The study concludes that siderophore activity could decrease the abundance of pathogenic bacteria and regulate the composition of the microbial community in TBW soil. The activities of sucrase (S-SC) and urease (S-UE) in TBW soil were significantly increased. Overall, the siderophore regulation of community structures is a sustainable management strategy for soil ecosystems.
微生物分泌的铁载体可提高生态效率，并可用于调节失衡的微生物群落结构。研究了云南木霉2-14F2菌株和拟布氏白僵菌（2-8F2）菌株的铁载体活性对受烟草青枯病（TBW）影响的土壤微生物生理生化功能及群落结构的影响。采用DNS比色法和Biolog-eco平板法量化菌株铁载体对土壤酶活性和微生物代谢的影响。基于Illumina MiSeq高通量测序，扩增土壤16S rDNA和ITS序列，剖析土壤微生物群落的α/β多样性以及结构/组成对铁载体的响应特征。利用KEGG数据库对微生物群落进行PICRUSt功能预测。我们发现，2-14F2和2-8F2的铁载体在一定浓度下，显著提高了TBW土壤中蔗糖酶（S-SC）和脲酶（S-UE）的活性，并增强了微生物群落的平均孔颜色发展（AWCD，碳源利用能力）。患病土壤对氨基酸、碳水化合物、聚合物、芳烃和羧酸的代谢能力也显著增强。细菌群落对铁载体活性代谢产物的响应在α多样性方面更为显著，而真菌群落的β多样性对铁载体的响应更为积极。 、 和 的相对丰度增加，同时 和 减少。LEfSe分析表明，在不同浓度的铁载体活性代谢产物作用下， 、 、 、 和 的变化最为明显。PICRUSt功能预测结果表明，铁载体增加了TBW土壤中微生物群落氧化还原相关酶的丰度。BugBase表型预测结果表明，铁载体活性可降低病原菌的丰度。研究得出结论，铁载体活性可降低病原菌的丰度，并调节TBW土壤中微生物群落的组成。TBW土壤中蔗糖酶（S-SC）和脲酶（S-UE）的活性显著提高。总体而言，铁载体对群落结构的调节是土壤生态系统的一种可持续管理策略。
