State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Science, Hubei University, Wuhan, 430062, China.
Tobacco Research Institute of Hubei Province, Wuhan, 430030, China.
Sci Rep. 2021 Jan 29;11(1):2653. doi: 10.1038/s41598-021-82060-3.
Bacterial wilt as a soil-borne disease was caused by Ralstonia solanacearum, and seriously damages the growth of tobacco. Integrated biocontrol method was explored to control bacterial wilt. Nevertheless, the long-term effects of the integrated biocontrol method on soil bacterial community, soil physicochemical properties and the incidence of bacterial wilt are not well understood. In this study, B. amyoliquefaciens ZM9, calcium cyanamide and rice bran were applied to tobacco fields in different ways. The disease index and incidence of tobacco bacterial wilt (TBW), soil physicochemical properties, colonization ability of B. amyoliquefaciens ZM9, and rhizopshere bacterial community were investigated. The results showed that the integrated application of B. amyoliquefaciens ZM9, rice bran and calcium cyanamide had the highest control efficiency of TBW and bacteria community diversity. Additionally, the integrated biocontrol method could improve the colonization ability of B. amyoliquefaciens ZM9. Furthermore, the integrated biocontrol method could effectively suppress TBW by regulating soil physicochemical properties, promoting beneficial bacteria and antagonistic bacteria of rhizopshere soil. This strategy has prospect of overcoming the defects in application of a single antagonistic bacteria and provides new insights to understand how to improve the colonization capacity of antagonistic bacteria and control efficacy for TBW.
细菌性萎蔫病是一种土传病害，由茄科劳尔氏菌引起，严重损害烟草的生长。本研究采用枯草芽孢杆菌 ZM9、石灰氮和米糠，以不同的方式应用于烟草田，以探索综合生物防治方法对土壤细菌群落、土壤理化性质和烟草细菌性萎蔫病（TBW）发病率的长期影响。结果表明，枯草芽孢杆菌 ZM9、米糠和石灰氮的综合应用对 TBW 和细菌群落多样性具有最高的防治效率。此外，综合生物防治方法可以提高枯草芽孢杆菌 ZM9 的定殖能力。此外，综合生物防治方法可以通过调节土壤理化性质、促进根际土壤有益细菌和拮抗菌的生长来有效抑制 TBW。该策略有望克服单一拮抗菌应用的缺陷，为提高拮抗菌的定殖能力和 TBW 防治效果提供新的思路。
