孟加拉国牲畜中[具体物种1]属和[具体物种2]属的分子检测与系统发育分析

Molecular Detection and Phylogenetic Analyses of spp. and spp. in Livestock in Bangladesh.

作者信息

Mohanta Uday Kumar, Chikufenji Boniface, Galon Eloiza May, Ji Shengwei, Ma Zhuowei, El-Sayed Shimaa Abd El-Salam, Ringo Aaron Edmond, Do Thanh Thom, Xuan Xuenan

机构信息

National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Hokkaido, Japan.

Department of Microbiology and Parasitology, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka 1207, Bangladesh.

出版信息

Microorganisms. 2023 Jun 13;11(6):1563. doi: 10.3390/microorganisms11061563.

Piroplasmosis, caused by spp. and spp., poses significant constraints for livestock production and upgradation in Bangladesh. Besides examining blood smears, few molecular reports are available from some selected areas in the country. Therefore, the actual scenario of piroplasmosis in Bangladesh is deficient. This study aimed to screen the piroplasms in different livestock species by molecular tools. A total of 276 blood samples were collected from cattle (), gayals () and goats () in five geographies of Bangladesh. After that, screening was conducted through a polymerase chain reaction, and species were confirmed by sequencing. The prevalence of , , , , and was 49.28%, 0.72%, 1.09%, 32.26%, 6.52% and 46.01%, respectively. The highest prevalence (79/109; 72.48%) of co-infections was observed with and . The phylogenetic analyses revealed that the sequences of (), (), (), (ssu rRNA) and () were included in one clade in the respective phylograms. In contrast, (MPSP) sequences were separated into two clades, corresponding to Types 5 and 7. To our knowledge, this is the first molecular report on piroplasms in gayals and goats in Bangladesh.

由巴贝斯虫属（Babesia spp.）和泰勒虫属（Theileria spp.）引起的梨形虫病，对孟加拉国的畜牧业生产和升级构成了重大限制。除了检查血涂片外，该国一些特定地区的分子报告很少。因此，孟加拉国梨形虫病的实际情况并不明晰。本研究旨在通过分子工具筛查不同家畜物种中的梨形虫。总共从孟加拉国五个地区的牛（）、大额牛（）和山羊（）身上采集了276份血样。之后，通过聚合酶链反应进行筛查，并通过测序确认物种。巴贝斯虫属、泰勒虫属、边缘无浆体（Anaplasma marginale）、嗜吞噬细胞无形体（Anaplasma phagocytophilum）、牛支原体（Mycoplasma bovis）和瑟氏泰勒虫（Theileria sergenti）的患病率分别为49.28%、0.72%、1.09%、32.26%、6.52%和46.01%。共感染的最高患病率（79/109；72.48%）出现在巴贝斯虫属和瑟氏泰勒虫之间。系统发育分析表明，巴贝斯虫属（）、泰勒虫属（）、边缘无浆体（）、嗜吞噬细胞无形体（，小亚基核糖体RNA）和瑟氏泰勒虫（）的序列在各自的系统发育图中被归入一个分支。相比之下，瑟氏泰勒虫（MPSP）序列被分为两个分支，分别对应5型和7型。据我们所知，这是孟加拉国关于大额牛和山羊梨形虫的第一份分子报告。