National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Hokkaido, Japan; Department of Microbiology and Parasitology, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka 1207, Bangladesh.
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Hokkaido, Japan.
Parasitol Int. 2023 Dec;97:102790. doi: 10.1016/j.parint.2023.102790. Epub 2023 Aug 5.
The emergence of Tick-borne Anaplasma spp. poses a significant threat to humans and animals worldwide. Traditional surveys based on examining blood smears overlook the existence of emerging pathogens. This study aimed to screen Anaplasma spp. in livestock species from diverse geographies with molecular tools. We collected 276 blood samples from cattle (Bos indicus), gayals (Bos frontalis) and goats (Capra hircus) in Jhenaidah, Bogura, Sirajganj and Bandarban districts, and Naikhongchari sub-district from June 2021 to March 2022. After that, a molecular screening was conducted through polymerase chain reaction (PCR) and sequencing was done to confirm the PCR results. The PCR assays were performed based on the analyses of groEL (Anaplasma marginale) and 16S rRNA (A. phagocytophilum and A. bovis). The Anaplasma spp. detected in this study were A. marginale (10.51%), A. phagocytophilum (0.72%), and A. bovis (63.77%). However, A. platys was not detected in this study. Among the screened pathogens, the detection of A. bovis (82.86%) was significantly high in the Bandarban district, while A. marginale was found only in cattle in this location. Regarding animal species, the occurrence of A. bovis was significantly higher in cattle. Moreover, the detection rate of A. marginale was significantly higher in adult cattle (≥2 years). The phylogenetic analyses revealed that the groEL sequences of A. marginale and 16S rRNA sequences of A. bovis and A. phagocytophilum were included in a single clade in the respective phylograms, showing a single genotype of each species circulating in Bangladesh. This study reports the existence of A. phagocytophilum in Bangladesh for the first time.
蜱传无形体属的出现对全球人类和动物构成了重大威胁。基于血涂片检查的传统调查方法忽略了新兴病原体的存在。本研究旨在使用分子工具筛选来自不同地理位置的牲畜中的无形体属。我们于 2021 年 6 月至 2022 年 3 月在杰纳伊达、波古拉、锡拉杰甘杰和班达班以及瑙因恰里分区采集了来自印度野牛（Bos indicus）、大额牛（Bos frontalis）和山羊（Capra hircus）的 276 份血液样本。之后，我们通过聚合酶链反应（PCR）进行了分子筛选，并对 PCR 结果进行了测序确认。PCR 检测基于 groEL（边缘无形体）和 16S rRNA（嗜吞噬无形体和牛无形体）的分析进行。本研究中检测到的无形体属包括边缘无形体（10.51%）、嗜吞噬无形体（0.72%）和牛无形体（63.77%）。然而，本研究未检测到平角无形体。在所筛选的病原体中，牛无形体（82.86%）在班达班地区的检出率显著较高，而在该地区仅在牛中发现边缘无形体。就动物物种而言，牛无形体在牛中的检出率显著较高。此外，成年牛（≥2 岁）中边缘无形体的检出率显著较高。系统发育分析显示，边缘无形体的 groEL 序列和牛无形体及嗜吞噬无形体的 16S rRNA 序列在各自的系统发育树中均包含在单个分支内，表明在孟加拉国流行的每个物种均为单一基因型。本研究首次报告了嗜吞噬无形体在孟加拉国的存在。
