Liao Chen-Chieh, Chen Chun-Hsien, Shin Jyh-Wei, Lin Wei-Chen, Chen Chien-Chin, Chu Chun-Ting
Department of Parasitology, Institute of Microbiology and Immunology, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan.
Department of Physiology, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan.
Microorganisms. 2023 Jun 14;11(6):1582. doi: 10.3390/microorganisms11061582.
is an intestinal protozoan that is often neglected, despite causing abdominal pain and diarrhea. Previous research has demonstrated that lipids can be synthesized by or can accumulate in growth medium, but their function and mechanisms in the pathogenesis of remain unclear. Our study found that lipid-rich ST7-B can increase inflammation and disrupt Caco-2 cells more than the same parasite without the lipovenoes supplement. Additionally, the cysteine protease of , a virulence factor, is upregulated and has higher activity in lipid-rich . In order to better understand the effects of lipids on pathogenesis, we treated lipid-lowering pravastatin during ST7-B culturing with a lipovenoes supplement, which decreased the lipid levels of the and reduced the -induced inflammation and cell disruption of Caco-2 cells. We also analyzed the fatty acid composition and possible synthesis pathway in ST7-B, finding significantly higher ratios of arachidonic acid, oleic acid, and palmitic acid than in the other lipid components in lipid-rich ST7-B. These results suggest that lipids play a significant role in the pathogenesis of and provide important information on the molecular mechanisms of and potential treatments for infection.
是一种常被忽视的肠道原生动物，尽管它会引起腹痛和腹泻。先前的研究表明，脂质可由其合成或可在生长培养基中积累，但其在发病机制中的功能和机制仍不清楚。我们的研究发现，富含脂质的ST7 - B比没有脂质补充剂的相同寄生虫更能增加炎症并破坏Caco - 2细胞。此外，作为一种毒力因子的半胱氨酸蛋白酶在富含脂质的ST7 - B中上调且具有更高的活性。为了更好地理解脂质对发病机制的影响，我们在富含脂质补充剂的ST7 - B培养过程中用降脂药物普伐他汀进行处理，这降低了ST7 - B的脂质水平，并减少了其诱导的Caco - 2细胞炎症和细胞破坏。我们还分析了ST7 - B中的脂肪酸组成和可能的合成途径，发现富含脂质的ST7 - B中花生四烯酸、油酸和棕榈酸的比例显著高于其他脂质成分。这些结果表明脂质在发病机制中起重要作用，并为感染的分子机制和潜在治疗提供了重要信息。
