Kaczmarek Adam, Gołąb Elżbieta, Żarnowska-Prymek Hanna, Rawska Anna, Jańczak Dawid, Lewicki Artur, Wesołowska Maria, Rożej-Bielicka Wioletta, Cielecka Danuta, Sałamatin Rusłan
National Institute of Public Health – National Institute of Hygiene, Warsaw, Poland, Department of Parasitology
Hospital of Infectious Diseases in Warsaw, Poland, Parasitology Laboratory
Przegl Epidemiol. 2017;71(4):539-546. Epub 2018 Feb 7.
Blastocystis hominis s. l. is one of the most commonly detected protozoa in the human large intestine. The aim of the study was to determine the genetic subtypes of Blastocystis hominis s. l. occurring in humans in Poland.
Stool samples from patients diagnosed in the Laboratory of the Department of Parasitology, National Institute of Public Health – National Institute of Hygiene (NIZP-PZH) and in the Parasitology Laboratory of the Hospital for Infectious Diseases in Warsaw were examined. Blastocystis subtypes were assayed based on the fragment of small-subunit ribosomal RNA gene sequences (SSU rDNA).
The examined isolates were classified into five Blastocystis subtypes (STs), fifteen of which belonged to ST3, three to ST1, two to ST2, two to ST6, and one isolate belonged to ST7. In three cases the subtype of isolate was not identified.
In Poland, the subtypes ST1, ST2, ST3, ST4, ST6 and ST7 have been reported in humans so far. The ST6 and ST7 subtypes are rarely detected in humans in Europe. In Poland, the ST6 subtype was previously described in chickens. On the basis of the studies, it was found that Blastocystis isolated from humans in Warsaw show high genetic diversity. In order to determine the possible pathogenic potential of individual Blastocystis subtypes, special epidemiological studies are required.
人芽囊原虫（Blastocystis hominis s. l.）是在人类大肠中最常检测到的原生动物之一。本研究的目的是确定在波兰人群中出现的人芽囊原虫的基因亚型。
对在国家公共卫生研究所 - 国家卫生研究所（NIZP - PZH）寄生虫学实验室以及华沙传染病医院寄生虫学实验室诊断的患者粪便样本进行检测。基于小亚基核糖体RNA基因序列（SSU rDNA）片段对芽囊原虫亚型进行分析。
所检测的分离株被分为五种芽囊原虫亚型（STs），其中15株属于ST3，3株属于ST1，2株属于ST2，2株属于ST6，1株属于ST7。有3例分离株的亚型未被鉴定。
在波兰，迄今为止已在人类中报告了ST1、ST2、ST3、ST4、ST6和ST7亚型。ST6和ST7亚型在欧洲人群中很少被检测到。在波兰，之前在鸡中描述过ST6亚型。基于这些研究，发现从华沙人类分离出的芽囊原虫具有高度的遗传多样性。为了确定各个芽囊原虫亚型可能的致病潜力，需要进行特殊的流行病学研究。
