Gong Biran, Zhao Lei, Zeng Chunyan, Zhu Wei, Xu Lili, Wu Dandan, Cheng Yiran, Wang Yi, Zeng Jian, Fan Xing, Sha Lina, Zhang Haiqin, Chen Guoyue, Zhou Yonghong, Kang Houyang
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, China.
Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, China.
Plants (Basel). 2023 Jun 14;12(12):2311. doi: 10.3390/plants12122311.
Stripe rust, which is caused by f. sp. , is one of the most devastating foliar diseases of common wheat worldwide. Breeding new wheat varieties with durable resistance is the most effective way of controlling the disease. Tetraploid (2n = 4x = 28, EEEE) carries a variety of genes conferring resistance to multiple diseases, including stripe rust, Fusarium head blight, and powdery mildew, which makes it a valuable tertiary genetic resource for enhancing wheat cultivar improvement. Here, a novel wheat-tetraploid 6E (6D) disomic substitution line (K17-1065-4) was characterized using genomic in situ hybridization and fluorescence in situ hybridization chromosome painting analyses. The evaluation of disease responses revealed that K17-1065-4 is highly resistant to stripe rust at the adult stage. By analyzing the whole-genome sequence of diploid , we detected 3382 specific SSR sequences on chromosome 6E. Sixty SSR markers were developed, and thirty-three of them can accurately trace chromosome 6E of tetraploid which were linked to the disease resistance gene(s) in the wheat genetic background. The molecular marker analysis indicated that 10 markers may be used to distinguish from other wheat-related species. Thus, K17-1065-4 carrying the stripe rust resistance gene(s) is a novel germplasm useful for breeding disease-resistant wheat cultivars. The molecular markers developed in this study may facilitate the mapping of the stripe rust resistance gene on chromosome 6E of tetraploid .
条锈病由小麦条锈菌引起，是全球普通小麦最具毁灭性的叶部病害之一。培育具有持久抗性的小麦新品种是控制该病的最有效方法。四倍体（2n = 4x = 28，EEEE）携带多种抗多种病害的基因，包括条锈病、赤霉病和白粉病，这使其成为增强小麦品种改良的宝贵三级遗传资源。在此，利用基因组原位杂交和荧光原位杂交染色体涂染分析对一个新型小麦-四倍体6E（6D）二体代换系（K17-1065-4）进行了鉴定。病害反应评估表明，K17-1065-4在成株期对条锈病高度抗性。通过分析二倍体的全基因组序列，我们在6E染色体上检测到3382个特异SSR序列。开发了60个SSR标记，其中33个能在小麦遗传背景中准确追踪四倍体的6E染色体，这些染色体与抗病基因连锁。分子标记分析表明，10个标记可用于区分与其他小麦相关物种。因此，携带条锈病抗性基因的K17-1065-4是一种用于培育抗病小麦品种的新型种质。本研究开发的分子标记可能有助于四倍体6E染色体上条锈病抗性基因的定位。
