Crop Development Centre and Department of Plant Sciences, University of Saskatchewan, Saskatoon, SK, S7N 5A8, Canada.
Institute of Evolution, University of Haifa, 199 Abba-Hushi Avenue, Mt. Carmel, 3498838, Haifa, Israel.
Commun Biol. 2022 Aug 17;5(1):826. doi: 10.1038/s42003-022-03773-3.
Durable crop disease resistance is an essential component of global food security. Continuous pathogen evolution leads to a breakdown of resistance and there is a pressing need to characterize new resistance genes for use in plant breeding. Here we identified an accession of wild emmer wheat (Triticum turgidum ssp. dicoccoides), PI 487260, that is highly resistant to multiple stripe rust isolates. Genetic analysis revealed resistance was conferred by a single, incompletely dominant gene designated as Yr84. Through bulked segregant analysis sequencing (BSA-Seq) we identified a 52.7 Mb resistance-associated interval on chromosome 1BS. Detected variants were used to design genetic markers for recombinant screening, further refining the interval of Yr84 to a 2.3-3.3 Mb in tetraploid wheat genomes. This interval contains 34 candidate genes encoding for protein domains involved in disease resistance responses. Furthermore, KASP markers closely-linked to Yr84 were developed to facilitate marker-assisted selection for rust resistance breeding.
持久的作物抗病性是全球粮食安全的重要组成部分。病原体的持续进化导致了抗性的丧失，因此迫切需要鉴定新的抗性基因，用于植物育种。在这里，我们鉴定了一个野生二粒小麦（Triticum turgidum ssp. dicoccoides）品系 PI 487260，它对多种条锈菌分离物具有高度抗性。遗传分析表明，抗性由一个单基因控制，该基因被命名为 Yr84，表现为不完全显性。通过 bulked segregant analysis sequencing (BSA-Seq)，我们在 1BS 染色体上鉴定到一个 52.7Mb 的与抗性相关的区间。检测到的变异被用于设计遗传标记进行重组筛选，进一步将 Yr84 的区间精确定位到四倍体小麦基因组中的 2.3-3.3Mb。该区间包含 34 个候选基因，编码参与抗病反应的蛋白结构域。此外，还开发了与 Yr84 紧密连锁的 KASP 标记，以促进锈病抗性育种的标记辅助选择。
