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利用ND-FISH和GISH技术对种间杂交种与野生物种之间的子代进行染色体分析。

Chromosomal analysis of progenies between intersectional hybrids and wild species using ND-FISH and GISH.

作者信息

Zhou Mei, Yong Xue, Zhu Jungang, Xu Qian, Liu Xiaodan, Zhang Lu, Mou Lisha, Zeng Lijia, Wu Mengxi, Jiang Beibei, Jia Yin, Zhang Peihua, Pan Yuanzhi

机构信息

College of Landscape Architecture, Sichuan Agricultural University, Chengdu, China.

Chengdu Botanical Garden, Chengdu, Sichuan, China.

出版信息

Front Plant Sci. 2024 Oct 22;15:1461798. doi: 10.3389/fpls.2024.1461798. eCollection 2024.

DOI:10.3389/fpls.2024.1461798
PMID:39502926
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11534598/
Abstract

INTRODUCTION

Intersectional hybrids in lilies possess significant breeding value, but the lack of complete lily genomes and complex genotypes pose challenges for early identification of lily hybrids. This study aimed to use intersectional hybrid cultivars as female parents and wild lilies as male parents to facilitate early identification of hybrid offsprings and enhance the efficiency and convenience of the process.

METHODS

We investigated the nature of cross combinations using Non-denaturing Fluorescence In Situ Hybridization (ND-FISH) and Genomic In Situ Hybridization (GISH) techniques. Three novel oligonucleotide probes-Oligo-pTa794, Oligo-pITS and Oligo-telo-were developed for lily chromosome research.

RESULTS

Our results demonstrated successful hybridization between wild lilies and intersectional hybrid cultivars, producing a total of 130 hybrid progenies. The combination of ND-FISH and GISH analyses effectively revealed the genomic composition of the hybrid progeny and determined the parental origin of specific chromosomes.

DISCUSSION

This research provides significant guidance for lily breeding practices and offers a valuable reference for the application of ND-FISH and GISH techniques in interspecific hybridization breeding and molecular cytogenetic research across various plant species. The methods developed enable more precise, efficient, and convenient identification of hybrid offsprings.

摘要

引言

百合的种间杂种具有重要的育种价值，但百合完整基因组的缺乏和复杂的基因型给百合杂种的早期鉴定带来了挑战。本研究旨在以种间杂交品种为母本，野生百合为父本，以促进杂种后代的早期鉴定，提高该过程的效率和便利性。

方法

我们使用非变性荧光原位杂交（ND-FISH）和基因组原位杂交（GISH）技术研究杂交组合的性质。开发了三种用于百合染色体研究的新型寡核苷酸探针——Oligo-pTa794、Oligo-pITS和Oligo-telo。

结果

我们的结果表明野生百合与种间杂交品种成功杂交，共产生了130个杂种后代。ND-FISH和GISH分析相结合有效地揭示了杂种后代的基因组组成，并确定了特定染色体的亲本来源。

讨论

本研究为百合育种实践提供了重要指导，并为ND-FISH和GISH技术在各种植物物种的种间杂交育种和分子细胞遗传学研究中的应用提供了有价值的参考。所开发的方法能够更精确、高效和方便地鉴定杂种后代。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bad0/11534598/bdd0674c8705/fpls-15-1461798-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bad0/11534598/665624b9434b/fpls-15-1461798-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bad0/11534598/919b73bf97b3/fpls-15-1461798-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bad0/11534598/e1a9b93d6f68/fpls-15-1461798-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bad0/11534598/672ef469ce53/fpls-15-1461798-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bad0/11534598/c0a7738a141c/fpls-15-1461798-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bad0/11534598/cb1653d9655c/fpls-15-1461798-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bad0/11534598/fcda46e40281/fpls-15-1461798-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bad0/11534598/bdd0674c8705/fpls-15-1461798-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bad0/11534598/665624b9434b/fpls-15-1461798-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bad0/11534598/919b73bf97b3/fpls-15-1461798-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bad0/11534598/e1a9b93d6f68/fpls-15-1461798-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bad0/11534598/672ef469ce53/fpls-15-1461798-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bad0/11534598/c0a7738a141c/fpls-15-1461798-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bad0/11534598/cb1653d9655c/fpls-15-1461798-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bad0/11534598/fcda46e40281/fpls-15-1461798-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bad0/11534598/bdd0674c8705/fpls-15-1461798-g008.jpg

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