Rambau Unarine, Naidoo Yougasphree, Sadashiva Channangihalli Thimmegowda, Baijnath Himansu, Dewir Yaser Hassan, Magyar-Tábori Katalin
School of Life Sciences, University of KwaZulu-Natal, Westville Campus, Private Bag X54001, Durban 4000, South Africa.
Plant Production Department, College of Food and Agriculture Sciences, King Saud University, Riyadh 11451, Saudi Arabia.
Plants (Basel). 2023 Jun 14;12(12):2312. doi: 10.3390/plants12122312.
(Harv.) Desc. ex Wild & R.B. Drumm is a perennial climber, indigenous to Southern Africa, and belongs to the Vitaceae. Although there have been many studies of Vitaceae micromorphology, only a few taxa have been described in detail. This study aimed to characterize the micro-morphology of the leaf indumentum and determining its possible functions. Stereo microscope, scanning electron microscope (SEM), and transmission electron microscope (TEM) were used to produce images. Micrographs of stereomicroscopy and SEM showed the presence of non-glandular trichomes. In addition, pearl glands were observed on the abaxial surface using a stereo microscope and SEM. These were characterized by a short stalk and a spherical- shaped head. The density of trichomes decreased on both surfaces of leaves as the leaf expanded. Idioblasts that contained raphide crystals were also detected in tissues. The results obtained from various microscopy techniques confirmed that non-glandular trichomes serve as the main external appendages of the leaves. Additionally, their functions may include serving as a mechanical barrier against environmental factors such as low humidity, intense light, elevated temperatures, as well as herbivory and insect oviposition. Our results may also be added to the existing body of knowledge with regard to microscopic research and taxonomic applications.
（哈夫.）由怀尔德和R.B. 德拉姆描述的该植物是一种多年生攀缘植物，原产于南部非洲，属于葡萄科。尽管对葡萄科的微形态学已有许多研究，但只有少数分类群得到了详细描述。本研究旨在描述叶片表皮毛的微观形态并确定其可能的功能。使用体视显微镜、扫描电子显微镜（SEM）和透射电子显微镜（TEM）来生成图像。体视显微镜和SEM的显微照片显示存在非腺毛。此外，使用体视显微镜和SEM在叶片背面观察到了珍珠状腺体。这些腺体的特征是有短柄和球形头部。随着叶片展开，叶片两面的毛密度降低。在组织中还检测到含有针晶的异细胞。从各种显微镜技术获得的结果证实，非腺毛是叶片的主要外部附属物。此外，它们的功能可能包括作为抵御低湿度、强光、高温等环境因素以及食草动物和昆虫产卵的机械屏障。我们的结果也可能会补充到有关微观研究和分类学应用的现有知识体系中。
