种子提取物在代谢组学和计算研究的支持下展现出体外和体内抗阿尔茨海默病的潜力。
Seed Extract Exhibits In Vitro and In Vivo Anti-Alzheimer's Potential Supported by Metabolomic and Computational Investigation.
作者信息
Bakhsh Hussain T, Mokhtar Fatma A, Elmaidomy Abeer H, Aly Hanan F, Younis Eman A, Alzubaidi Mubarak A, Altemani Faisal H, Algehainy Naseh A, Majrashi Mohammed Ali A, Alsenani Faisal, Bringmann Gerhard, Abdelmohsen Usama Ramadan, Abdelhafez Omnia Hesham
机构信息
Department of Pharmacy Practice, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
Department of Pharmacognosy, Faculty of Pharmacy, El Saleheya El Gadida University, El Saleheya El Gadida, Sharkia 44813, Egypt.
出版信息
Plants (Basel). 2023 Jun 20;12(12):2382. doi: 10.3390/plants12122382.
Linn. (okra, F. Malvaceae) is a fruit widely consumed all over the world. In our study, the anti-Alzheimer's potential of was evaluated. An in vitro DPPH free radical assay on seed's total extract and AChE inhibition potential screening indicated a significant anti-Alzheimer's activity of the extract, which was confirmed through an in vivo study in an aluminum-intoxicated rat model. Additionally, in vivo results demonstrated significant improvement in Alzheimer's rats, which was confirmed by improving T-maze, beam balance tests, lower serum levels of AChE, norepinephrine, glycated end products, IL-6, and MDA. The levels of dopamine, BDNF, GSH, and TAC returned to normal values during the study. Moreover, histological investigations of brain tissue revealed that the destruction in collagen fiber nearly returns back to the normal pattern. Metabolomic analysis of the ethanolic extract of seeds via LC-HR-ESI-MS dereplicated ten compounds. A network pharmacology study displayed the relation between identified compounds and 136 genes, among which 84 genes related to Alzheimer's disorders, and focused on AChE, APP, BACE1, MAPT and TNF genes with interactions to all Alzheimer's disorders. Consequently, the results revealed in our study grant potential dietary elements for the management of Alzheimer's disorders.
林奈（秋葵，锦葵科）是一种在全球广泛食用的果实。在我们的研究中，对[具体名称未给出]的抗阿尔茨海默病潜力进行了评估。对[具体名称未给出]种子总提取物进行的体外DPPH自由基测定以及乙酰胆碱酯酶抑制潜力筛选表明该提取物具有显著的抗阿尔茨海默病活性，这在铝中毒大鼠模型的体内研究中得到了证实。此外，体内结果显示阿尔茨海默病大鼠有显著改善，这通过改善T迷宫、横梁平衡测试、降低血清中乙酰胆碱酯酶、去甲肾上腺素、糖化终产物、白细胞介素 - 6和丙二醛的水平得到了证实。在研究过程中，多巴胺、脑源性神经营养因子、谷胱甘肽和总抗氧化能力水平恢复到正常数值。此外，脑组织的组织学研究表明胶原纤维的破坏几乎恢复到正常模式。通过LC - HR - ESI - MS对[具体名称未给出]种子乙醇提取物进行代谢组学分析鉴定出了十种化合物。一项网络药理学研究展示了所鉴定化合物与136个基因之间的关系，其中84个基因与阿尔茨海默病相关疾病有关，并聚焦于与所有阿尔茨海默病相关疾病相互作用的乙酰胆碱酯酶、淀粉样前体蛋白、β - 分泌酶1、微管相关蛋白tau和肿瘤坏死因子基因。因此，我们研究中揭示的结果为阿尔茨海默病相关疾病的管理提供了潜在的膳食元素。
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